A practical and reliable procedure for in vitro induction of tetraploid tomato
| dc.contributor.author | Praça, Milene Miranda | |
| dc.contributor.author | Carvalho, Carlos Roberto de | |
| dc.contributor.author | Clarindo, Wellington Ronildo | |
| dc.date.accessioned | 2018-12-05T10:08:23Z | |
| dc.date.available | 2018-12-05T10:08:23Z | |
| dc.date.issued | 2009-10-01 | |
| dc.description.abstract | A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets. | en |
| dc.format | pt-BR | |
| dc.identifier.issn | 0304-4238 | |
| dc.identifier.uri | https://doi.org/10.1016/j.scienta.2009.05.032 | |
| dc.identifier.uri | http://www.locus.ufv.br/handle/123456789/22692 | |
| dc.language.iso | eng | pt-BR |
| dc.publisher | Scientia Horticulturae | pt-BR |
| dc.relation.ispartofseries | Volume 122, Issue 3, Pages 501- 505, October 2009 | pt-BR |
| dc.rights | 2009 Elsevier B.V. All rights reserved. | pt-BR |
| dc.subject | Colchicine | pt-BR |
| dc.subject | Chromosome | pt-BR |
| dc.subject | Flow cytometry | pt-BR |
| dc.subject | Solanum lycopersicum | pt-BR |
| dc.title | A practical and reliable procedure for in vitro induction of tetraploid tomato | en |
| dc.type | Artigo | pt-BR |