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https://locus.ufv.br//handle/123456789/21302
Tipo: | Artigo |
Título: | Evidence for the association of the human regulatory protein Ki-1/57 with the translational machinery |
Autor(es): | Gonçalves, Kaliandra de Almeida Bressan, Gustavo Costa Saito, Ângela Morello, Luis Gustavo Zanchin, Nilson Ivo T. Kobarg, Jörg |
Abstract: | Ki-1/57 is a cytoplasmic and nuclear protein of 57 kDa first identified in malignant cells from Hodgkin’s lymphoma. Based on yeast-two hybrid protein interaction we found out that Ki-1/57 interacts with adaptor protein RACK1 (receptor of activated kinase 1), CIRP (cold-inducible RNA-binding protein), RPL38 (ribosomal protein L38) and FXR1 (fragile X mental retardation-related protein 1). Since these proteins are involved in the regulation of translation we suspected that Ki-1/57 may have a role in it. We show by immunoprecipitation the association of Ki-1/57 with FMRP. Confocal microscopy revealed that Ki-1/57 colocalizes with FMRP/FXR1/2 to stress granules. Furthermore Ki-1/57 cosediments with free ribosomal particles and enhances translation, when tethered to a reporter mRNA, suggesting that Ki-1/57 may be involved in translational regulation. |
Palavras-chave: | Regulatory protein Protein–protein interaction Translation |
Editor: | FEBS Letters |
Tipo de Acesso: | Elsevier B.V. |
URI: | https://doi.org/10.1016/j.febslet.2011.07.010 http://www.locus.ufv.br/handle/123456789/21302 |
Data do documento: | 19-Ago-2011 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 695,06 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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