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https://locus.ufv.br//handle/123456789/19237
Tipo: | Artigo |
Título: | Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases |
Autor(es): | Pylro, Victor Satler Morais, Daniel Kumazawa Kalks, Karlos Henrique Martins Roesch, Luiz Fernando Wurdig Hirsch, Penny R. Tótola, Marcos Rogério Yotoko, Karla |
Abstract: | Controversy surrounding bacterial phylogenies has become one of the most important challenges for microbial ecology. Comparative analyses with nucleotide databases and phylogenetic reconstruction of the amplified 16S rRNA genes from DGGE (Denaturing Gradient Gel Electrophoresis) excised bands have been used by several researchers for the identification of organisms in complex samples. Here, we individually analyzed DGGE-excised 16S rRNA gene bands from 10 certified bacterial strains of different species, and demonstrated that this kind of approach can deliver erroneous outcomes to researchers, besides causing/emphasizing errors in public databases. |
Palavras-chave: | 16S rRNA gene PCR-DGGE bacteria phylogeny Public databases |
Editor: | Journal of Microbiological Methods |
Tipo de Acesso: | Elsevier B.V. |
URI: | https://doi.org/10.1016/j.mimet.2016.04.012 http://www.locus.ufv.br/handle/123456789/19237 |
Data do documento: | 22-Abr-2016 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 403,38 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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