Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/19134
Tipo: | Artigo |
Título: | Covalent immobilization of α-Galactosidase from Penicillium griseoroseum and its application in Oligosaccharides Hydrolysis |
Autor(es): | Falkoski, Daniel Luciano Guimarães, Valéria Monteze Queiroz, Marisa Vieira de Araújo, Elza Fernandes de Almeida, Maíra Nicolau de Barros, Everaldo Gonçalves de Rezende, Sebastião Tavares de |
Abstract: | Partially purified α-Galactosidase from Penicillium griseoroseum was immobilized onto modified silica using glutaraldehyde linkages. The effective activity of immobilized enzyme was 33%. Free and immobilized α-galactosidase showed optimal activity at 45 °C and pH values of 5 and 4, respectively. Immobilized α-galactosidase was more stable at higher temperatures and pH values. Immobilized α-galactosidase from P. griseoroseum maintained 100% activity after 24 h of incubation at 40 °C, while free enzyme showed only 32% activity under the same incubation conditions. Defatted soybean flour was treated with free and immobilized α-galactosidase in batch reactors. After 8 h of incubation, stachyose was completely hydrolyzed in both treatments. After 8 h of incubation, 39% and 70% of raffinose was hydrolyzed with free and immobilized α-galactosidase respectively. Immobilized α-galactosidase was reutilized eight times without any decrease in its activity. |
Palavras-chave: | Penicillium griseoroseum α-Galactosidase Enzyme immobilization Modified silica Raffinose oligosaccharides Soybean products |
Editor: | Applied Biochemistry and Biotechnology |
Tipo de Acesso: | Humana Press |
URI: | https://doi.org/10.1007/s12010-008-8387-9 http://www.locus.ufv.br/handle/123456789/19134 |
Data do documento: | 21-Out-2008 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | Texto completo | 348,38 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.