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https://locus.ufv.br//handle/123456789/12619
Tipo: | Artigo |
Título: | Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene |
Autor(es): | Ribon, Andréa de O.B. Ribeiro, João Batista Gonçalves, Daniel B. Queiroz, Marisa V. de Araújo, Elza F. de |
Abstract: | Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied. |
Palavras-chave: | Penicillium griseoroseum Polygalacturonase 5' upstream regulatory sequence Electrophoretic mobility shift assay |
Editor: | Genetics and Molecular Biology |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1590/S1415-47572009005000021 http://www.locus.ufv.br/handle/123456789/12619 |
Data do documento: | 25-Jul-2008 |
Aparece nas coleções: | Artigos |
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gmb-32-1-129.pdf | texto completo | 93,41 kB | Adobe PDF | Visualizar/Abrir |
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