Navegando por Autor "Brommonschenkel, Sérgio Hermínio"
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Item Influência do tratamento com etileno sobre o teor de sólidos solúveis e a cor de pimentas(Bragantia, 2008-10) Pereira, Giselda Maria; Finger, Fernando Luiz; Casali, Vicente Wagner Dias; Brommonschenkel, Sérgio HermínioEste trabalho teve como objetivo avaliar a variabilidade genética em frutos de Capsicum com relação ao acúmulo de sólidos solúveis totais, degradação de clorofila, síntese de carotenóides e tempo necessário para o completo amadurecimento de frutos, em resposta ao tratamento com ethephon. O tratamento com ethephon provocou alteração do conteúdo de sólidos solúveis totais em frutos dos acessos BGH 4366 (C. baccatum) e BGH 4708 (C. frutescens), enquanto os demais acessos não foram responsivos ao tratamento. O amadurecimento dos frutos foi caracterizado por decréscimo no conteúdo de clorofila e conteúdo de carotenóides, contudo, não foi detectada variabilidade genética entre os acessos para a degradação da clorofila e para a síntese de carotenóides, quando tratados com ethephon. O uso de ethephon acelerou o amadurecimento nos acessos BGH 4179 (C. frutescens), BGH 6029 (C. baccatum) e Ca 6 (C. annuum).Item Inheritance and genetic mapping of resistance to Asian soybean rust in cultivar TMG 803(Crop Breeding and Applied Biotechnology, 2014-08-04) Matsuo, Éder; Sediyama, Tuneo; Brommonschenkel, Sérgio Hermínio; Cruz, Cosme DamiãoThis study analyzed the inheritance and identified microsatellite markers linked to the resistance gene to Phakopsora pachyrhizi in soybean cultivar TMG 803. Hybridization between the cultivars TMG 803 and BRS Valiosa RR was performed to obtain F1 progenies and the F2 population. The response of the parents 'TMG 803' and 'BRS Valiosa RR' to P. pachyrhizi was, respectively, resistant and susceptible, and among the 116 F2 plants,93 were resistant and 23 susceptible, under natural infection and field conditions. It was found that the resistance of cultivar TMG 803 is controlled by one gene with complete dominance, mapped as resistance locus Rpp4 of linkage group G. Of the 16 tested, one microsatellite marker, sc21_3420, was completely linked to the resistance gene (distance 0.0cM) and the favorable allelic form was present in cultivar TMG 803, which may therefore be useful in assisted selection in segregating populations.Item Padrão de integração de pAN7-1 em mutantes de Magnaporthe grisea com patogenicidade alterada em arroz(Summa Phytopathologica, 2010-01) Queiroz, Marisa Vieira de; Marchi, Carlos Eduardo; Brommonschenkel, Sérgio Hermínio; Borges, Mírian de Freitas; Mizubuti, Eduardo Seiti G.Ensaios foram conduzidos para verificar a presença, o número de cópias e de sítios de integração de pAN7-1 no genoma de mutantes de M. grisea I-22 com patogenicidade alterada em arroz. Foram analisados T41, T93, T251 (gerados por mutagênese REMI) e T108 (oriundo de mutagênese convencional), os quais exibiram diferentes fenótipos mutantes. O DNA total desses mutantes foi submetido à reação em cadeia de polimerase (PCR) e às análises de hibridização com o vetor (Southern blot). A presença de pAN7-1 no genoma de todos os mutantes foi confirmada por PCR. Segundo as análises de Southern blot, T41 exibiu duas integrações do vetor, ambas na forma de cópia única. No genoma de T93 também foram detectados dois sítios de inserção de pAN7-1, um dos quais envolvendo múltiplas cópias do vetor. Os resultados indicaram a presença de apenas uma cópia do vetor em um único sítio nos genomas de T108 e T251. O padrão de integração em T251 foi o único a sugerir a ocorrência de evento REMI. As diferenças quanto ao tamanho dos fragmentos com homologia a pAN7-1 refletiram a possível aleatoriedade dos eventos de integração no genoma de M. grisea. Os resultados evidenciaram o potencial de REMI para a mutagênese insercional de M. grisea, quando conduzida com pAN7-1 e HindIIIItem The repertoire of effector candidates in Colletotrichum lindemuthianum reveals important information about Colletotrichum genus lifestyle(Applied Microbiology and Biotechnology, 2019) Queiroz, Casley Borges de; Correia, Hilberty L. Nunes; Santana, Mateus Ferreira; Batista, Diego Silva; Vidigal, Pedro M. Pereira; Brommonschenkel, Sérgio Hermínio; Queiroz, Marisa Vieira deThe fungus Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean (Phaseolus vulgaris), and anthracnose is one of the most devastating diseases of this plant species. However, little is known about the proteins that are essential for the fungus-plant interactions. Knowledge of the fungus’ arsenal of effector proteins is of great importance for understanding this pathosystem. In this work, we analyzed for the first time the arsenal of Colletotrichum lindemuthianum effector candidates (ClECs) and compared them with effector proteins from other species of the genus Colletotrichum, providing a valuable resource for studying the infection mechanisms of these pathogens in their hosts. Isolates of two physiological races (83.501 and 89 A2 2-3) of C. lindemuthianum were used to predict 353 and 349 ClECs, respectively. Of these ClECs, 63% were found to be rich in cysteine, have repetitive sequences of amino acids, and/or possess nuclear localization sequences. Several conserved domains were found between the ClECs. We also applied the effector prediction to nine species in the genus Colletotrichum, and the results ranged from 247 predicted effectors in Colletotrichum graminicola to 446 in Colletotrichum orbiculare. Twelve conserved domains were predicted in the effector candidates of all analyzed species of Colletotrichum. An expression analysis of the eight genes encoding the effector candidates in C. lindemuthianum revealed their induction during the biotrophic phase of the fungus on the bean.Item The transcriptional landscape of basidiosporogenesis in mature Pisolithus microcarpus basidiocarp(BMC Genomics, 2017-02-02) Pereira, Maíra de Freitas; Campos, André Narvaes da Rocha; Anastacio, Thalita Cardoso; Morin, Emmanuelle; Brommonschenkel, Sérgio Hermínio; Martin, Francis; Kohler, Annegret; Costa, Maurício DutraPisolithus microcarpus (Cooke & Massee) G. Cunn is a gasteromycete that produces closed basidiocarps in symbiosis with eucalypts and acacias. The fungus produces a complex basidiocarp composed of peridioles at different developmental stages and an upper layer of basidiospores free of the hyphae and ready for wind dispersal upon the rupture of the basidiocarp pellis. During basidiosporogenesis, a process that takes place inside the basidiocarp peridioles, a conspicuous reserve of fatty acids is present throughout development. While several previous studies have described basidiosporogenesis inside peridioles, very little is known about gene expression changes that may occur during this part of the fungal life cycle. The objective of this work was to analyze gene transcription during peridiole and basidiospore development, while focusing specifically on cell cycle progression and lipid metabolism. Throughout different developmental stages of the peridioles we analyzed, 737 genes were regulated between adjacent compartments (>5 fold, FDR-corrected p-value < 0.05) corresponding to 3.49% of the genes present in the P. microcarpus genome. We identified three clusters among the regulated genes which showed differential expression between the peridiole developmental stages and the basidiospores. During peridiole development, transcripts for proteins involved in cellular processes, signaling, and information storage were detected, notably those for coding transcription factors, DNA polymerase subunits, DNA repair proteins, and genes involved in chromatin structure. For both internal embedded basidiospores (hereto referred to as “Internal spores”, IS) and external free basidiospores (hereto referred to as “Free spores”, FS), upregulated transcripts were found to involve primary metabolism, particularly fatty acid metabolism (FA). High expression of transcripts related to β-oxidation and the glyoxylate shunt indicated that fatty acids served as a major carbon source for basidiosporogenesis. Our results show that basidiocarp formation in P. microcarpus involves a complex array of genes that are regulated throughout peridiole development. We identified waves of transcripts with coordinated regulation and identified transcription factors which may play a role in this regulation. This is the first work to describe gene expression patterns during basidiocarp formation in an ectomycorrhizal gasteromycete fungus and sheds light on genes that may play important roles in the developmental process.