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https://locus.ufv.br//handle/123456789/23920
Tipo: | Artigo |
Título: | Ethanol stress responses of Kluyveromyces marxianus CCT 7735 revealed by proteomic and metabolomic analyses |
Autor(es): | Alvim, Mariana Caroline Tocantins Vital, Camilo Elber Vieira, Nívea Moreira Silveira, Fernando Augusto da Balbino, Thércia Rocha Diniz, Raphael Hermano Santos Brito, Amanda Fernandes Bazzolli, Denise Mara Soares Silveira, Wendel Batista da Barros, Edvaldo Ramos, Humberto Josué de Oliveira |
Abstract: | Kluyveromyces marxianus CCT 7735 offers advantages to ethanol production over Saccharomyces cerevisiae, including thermotolerance and the ability to convert lactose to ethanol. However, its growth is impaired at high ethanol concentrations. Herein we report on the protein and intracellular metabolite profiles of K. marxianus at 1 and 4 h under ethanol exposure. The concentration of some amino acids, trehalose and ergosterol were also measured. We observed that proteins and metabolites from carbon pathways and translation were less abundant, mainly at 4 h of ethanol stress. Nevertheless, the concentration of some amino acids and trehalose increased at 8 and 12 h under ethanol stress, indicating an adaptive response. Moreover, our results show that the abundance of proteins and metabolites related to the oxidative stresses responses increased. The results obtained in this study provide insights into understanding the physiological changes in K. marxianus under ethanol stress, indicating possible targets for ethanol tolerant strains construction. |
Palavras-chave: | Amino acids Trehalose Ergosterol Membrane permeability Lactose |
Editor: | Antonie van Leeuwenhoek |
Tipo de Acesso: | Springer Nature Switzerland AG |
URI: | https://doi.org/10.1007/s10482-018-01214-y http://www.locus.ufv.br/handle/123456789/23920 |
Data do documento: | 2019 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 1,61 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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