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https://locus.ufv.br//handle/123456789/18384
Tipo: | Artigo |
Título: | Enzymatic production of bioactive docosahexaenoic acid phenolic ester |
Autor(es): | Roby, Mohamed H. Allouche, Ahmad Dahdou, Layal Castro, Vanessa C. De Silva, Paulo H. Alves da Targino, Brenda N. Huguet, Marion Paris, Cédric Chrétien, Françoise Guéant, Rosa-Maria Desobry, Stéphane Oster, Thierry Humeau, Catherine |
Abstract: | Docosahexaenoic acid (DHA) is increasingly considered for its health benefits. However, its use as functional food ingredient is still limited by its instability. In this work, we developed an efficient and solvent-free bioprocess for the synthesis of a phenolic ester of DHA. A fed-batch process catalyzed by Candida antarctica lipase B was optimised, leading to the production of 440 g/L vanillyl ester (DHA-VE). Structural characterisation of the purified product indicated acylation of the primary OH group of vanillyl alcohol. DHA-VE exhibited a high radical scavenging activity in acellular systems. In vivo experiments showed increased DHA levels in erythrocytes and brain tissues of mice fed DHA-VE-supplemented diet. Moreover, in vitro neuroprotective properties of DHA-VE were demonstrated in rat primary neurons exposed to amyloid-β oligomers. In conclusion, DHA-VE synergized the main beneficial effects of two common natural biomolecules and therefore appears a promising functional ingredient for food applications. |
Palavras-chave: | Antioxidant DHA Enzymatic esterification Neuroprotection Vanillyl alcohol |
Editor: | Food Chemistry |
Tipo de Acesso: | Elsevier Ltd. All rights reserved. |
URI: | https://doi.org/10.1016/j.foodchem.2014.09.028 http://www.locus.ufv.br/handle/123456789/18384 |
Data do documento: | 16-Set-2014 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 1,2 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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