Bastos, Matheus Silva eSouza, Luciana Ângelo deOnofre, Thiago SouzaAlmeida, Márcia Rogéria deBressan, Gustavo CostaFietto, Juliana Lopes RangelSilva Júnior, Abelardo2017-10-112017-10-112016-10-201678-8060http://dx.doi.org/10.1590/0074-02760160237http://www.locus.ufv.br/handle/123456789/12018Gene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.pdfengOpen accessLeishmania braziliensisGreen fluorescent proteinDrug screeningArtigo