Dias, E. S.Araújo, E. F.Guimarães, W. V.Muchovej, R. M. C.2018-04-172018-04-171996-04-1115730972https://doi.org/10.1007/BF00327726http://www.locus.ufv.br/handle/123456789/18767Experiments were performed with the mycorrhizal fungus Suillus granulatus to define the parameters for production and regeneration of protoplasts. Protoplasts were released at frequencies between 1 and 3×107/ml from mycelium 3 to 7 days old. The best osmotic stabilizer for protoplast release was MgSO4 (0.7 m). To optimize protoplast release and regeneration an enzyme (Novozym 234) concentration 1.7 mg/ml was chosen, with a digestion time of 1 to 2 h. Regenerated colonies formed mycorrhizae within 60 days after inoculation in Pinus caribaea var. hondurensis seedlings.pdfengRapid Science PublishersMycorrhizaeProtoplastsSuillusArtigo