Santana, Mateus FerreiraAraújo, Elza Fernandes deQueiroz, Marisa Vieira deSouza, Jorge Teodoro deMizubuti, Eduardo Seiti Gomide2019-01-032019-01-032012-111573-8469https://doi.org/10.1007/s10658-012-0031-4http://www.locus.ufv.br/handle/123456789/22902Moniliophthora perniciosa is a fungus that causes witches’ broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70 M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa.pdfengKNPV 2012RetrotransposonMoniliophthora perniciosaGenetic variabilityIRAPREMAPArtigo