Centro de Ciências Biológicas e da Saúde
URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11833
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107 resultados
Resultados da Pesquisa
Item A comparative study of the ovaries in some Brazilian bees (Hymenoptera; Apoidea)(Papéis Avulsos de Zoologia, 2004) Martins, Gustavo Ferreira; Serrão, José EduardoThe present paper concerns the morphological features of ovaries in 33 species of bees with different social behavior patterns. The ovaries of bees were examined under light microscope. They are polytrophic-meroistic ovaries formed for an anterior germarium and a basal vitellarium. The germarium houses the germ cells and in the vitellarium there are follicles arranged linearly. In general the follicle is constituted by a nutritive chamber (a cluster of nurse cells) and an oocyte chamber, both covered by a single epithelial layer of follicular cells. The number of ovarioles per ovary and the number of mature oocyte per ovary were analyzed. Measurements of ovariole length, oocyte size, oocyte width, follicular epithelial height and the intertegular distance were made to support the comparative study. Statistical analysis showed that representatives of Meliponini and Apini have the largest ovaries. On the other hand, in solitary bees were found the bigger oocytes. Furthermore, our results suggest that there is a tendency for increase in ovary size and ovariole number, with increasing level of sociality.Item Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil(Hoehnea, 2018-07) Guimarães-Cestaro, Lubiane; Luz, Cynthia Fernandes Pinto da; Serrão, José Eduardo; Message, Dejair; Martins, Marta Fonseca; Alves, Maria Luisa Teles Marques Florêncio; Seijo-Coello, María Carmen; Teixeira, Érica WeinsteinRoyal jelly may contain pollen grains and their presence can be used to determine the phytogeographical origin of the product. This study analyzed the phytogeographical origin of commercial royal jelly samples from São Paulo State, tested as part of the Brazilian Federal Inspection System (SIF), found to be contaminated with spores of the bacterium Paenibacillus larvae, that causes the American Foulbrood Disease. The pollen grains of Castanea had the highest total percentage, with lower percentages of Cirsium/Carduus, Cistus, Parthenocissus, Prunus, Quercus, Robinia, Scrophulariaceae, Taraxacum, Tilia, among others. This pollen spectrum is incompatible with royal jelly samples produced in Brazil. The pollen spectrum resembled that of an imported product, compatible with the Northern Hemisphere origin. Brazilian legislation does not require the phytogeographic origin of imported bee products to be analyzed by palynological procedures, but it is mandatory to have a certificate issued by the country of origin attesting the absence of pathogens, monitored with the objective of preventing the exotic diseases from entering Brazil. Palynology, therefore, proved to be fundamental in detecting imports of this contaminated batches.Item Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service(Revista Brasileira de Entomologia, 2018-07) Guimarães-Cestaro, Lubiane; Teixeira, Érica Weinstein; Alves, Maria Luisa Teles Marques Florêncio; Message, Dejair; Martins, Marta Fonseca; Luz, Cynthia Fernandes Pinto da; Serrão, José EduardoDue to their ecological and economic importance, honey bees have attracted much scientific attention, which has intensified due to the recent population decline of these insects in the several parts of the world. Among the factors related to these patterns, infection by pathogens are the most relevant, mainly because of the easy dissemination of these microorganisms. Although no zoonotic diseases are associated with these insects, the presence of infectious agents in bee products should still be considered because they play a role as disease dispersers, increasing the risk to animal health. Because of the possibility of dispersion of pathogens via bee products, this work aimed to identify the presence of spores of the pathogens Paenibacillus larvae, Ascosphaera apis and Nosema spp. in samples of honey, pollen and royal jelly that are registered with Brazil's Federal Inspection Service (S.I.F.) and commercially available in the state of São Paulo. Of the 41 samples of bee products analyzed, only one showed no contamination by any of these pathogens. N. ceranae and P. larvae had the highest prevalence considering all the samples analyzed (present in 87.80% and 85.37% of the total, respectively), with N. apis present in 26.83% and A. apis present in 73.17% of the samples. These results provide support for the formulation of government regulations for sanitary control of exotic diseases by preventing dispersion of pathogens, including through illegal importation, since local and international trade and the transfer of colonies between regions play important roles in the dispersion of these microorganisms.Item As espécies de ephemeroptera (insecta) registradas para o Brasil(Biota Neotropica, 2004) Salles, Frederico Falcão; Serrão, José Eduardo; Hubbard, Michael D.; Da-Silva, Elidiomar RibeiroUma lista da fauna de Ephemeroptera do Brasil, com todas as espécies, gêneros e famílias registrados para o país é apresentada, incluindo os estados para os quais as espécies estão reportadas assim como a bibliografia pertinente. Comentários acerca do estado atual do conhecimento da fauna brasileira também são tecidos. Até o presente momento 10 famílias, 63 gêneros e 166 espécies estão registrados. As famílias Baetidae e Leptophlebiidae compreendem mais de 50% de todos os registros, enquanto as regiões Norte e Sudeste são significativamente melhor estudadas que as demais.Item Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome(Genetics and Molecular Biology, 2013) Sousa, Cristina Soares de; Serrão, José Eduardo; Bonetti, Ana Maria; Amaral, Isabel Marques Rodrigues; Kerr, Warwick Estevam; Maranhão, Andréa Queiroz; Ueira-Vieira, CarlosThe insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees.Item Sarsina violascens spatial and temporal distributions affected by native vegetation strips in eucalyptus plantations(Pesquisa Agropecuária Brasileira, 2016-06) Zanuncio, José Cola; Tavares, Wagner de Souza; Ramalho, Francisco de Sousa; Leite, Germano Leão Demolin; Serrão, José EduardoThe objective of this work was to evaluate spatial and temporal distributions of Sarsina violascens (Lepidoptera: Noctuidae: Lymantriinae) in two Eucalyptus cloeziana plantations, one with native vegetation strips (WNVS) and another without them (ONVS). Adults were collected with light traps, which were installed: inside an area of native vegetation (Cerrado), 100 m from the edge; at the contact zone between the native vegetation area and the E. cloeziana plantation; inside the E. cloeziana plantation, 250 m from the edge; at the central part of the native vegetation strip, around 500 m from the edge (WNVS) or in the contact zone between two E. cloeziana compartments (ONVS); and inside the E. cloeziana plantation, 500 m from the edge. The number of S. violascens adults collected was 240 in the system WNVS and 1,378 in the system ONVS. The lower number of individuals in the system WNVS was probably due to favored biological control provided by higher species richness with the use of native vegetation strips. These strips, intermingled with E. cloeziana plantations, allow a higher proportion of native forest in the landscape and can help to reduce S. violascens infestations.Item Post-embryonic development of intramandibular glands of Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae) workers(Papéis Avulsos de Zoologia, 2017) Oliveira, André Henrique de; Lisboa, Luciane Cristina de Oliveira; Serrão, José Eduardo; Zanuncio, José Cola; Souza, Edmilson Amaral deExocrine glands play important role in social organization of insects, such as caste and inter-caste differentiation. Due their functional and structural plasticity, morphological studies on such glands contribute to better understanding the biology of social bees. Therefore, the aim of the study was to characterize the sequence of the post-embryonic development of intramandibular glands of Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae) workers using histological and histochemical analyses. The mandibles of pupae at different developmental stages and newly emerged adults were analyzed. The intramandibular glands of F. schrottkyi presented two types: class I glands, in the mandible epidermis and class III glands, inside the mandible cavity that open onto external surface. The intramandibular glands of F. schrottkyi developed during the transition from the prepupae to the white-eyed pupae, as shown by the morphological changes. Black-eyed pupae of F. schrottkyi presented fully developed intramandibular glands.Item Pukuakanga salignea, gênero novo e espécie nova de Dictyopharidae (Hemiptera: Auchenorrhyncha: Fulgoromorpha) do Sudeste do Brasil(Biota Neotropica, 2010-10) Baptista, Marcelo da Silva; Serrão, José Eduardo; Da-Silva, Elidiomar RibeiroPukuakanga gen.nov. (Hemiptera: Dyctiopharidae) é erigido com base em exemplares adultos de uma espécie nova, Pukuakanga salignea sp.nov., provenientes de diferentes municípios do Estado de Minas Gerais, Brasil. O gênero pode ser determinado por uma combinação de características como: cabeça com o comprimento 4,8× a largura da base; ápice da projeção da cabeça apresentando a união das carenas marginais da fronte e do vértice lateralmente a projeção; ausência de carena na tégula; margem anteromediana do pronoto reta; três fileiras de células na membrana da asa anterior; edeago com a conjuntiva bifurcada formando dois pares de tubos e o espinho do pênis apresentando um ramo lateral.Item Development and survival of nymphs of Podisus nigrispinus (Heteroptera: Pentatomidae) fed with caterpillars of Chlosyne lacinia saundersii (Lepidoptera: Nymphalidae)(Brazilian Archives of Biology and Technology, 2009-01) Silva, Carlos Alberto Domingues da; Zanuncio, Teresinha Vinha; Cunha, Bruno Gomes; Castro, Ancidériton Antonio de; Canevari, Glauco da Cruz; Serrão, José Eduardo; Zanuncio, José ColaThe development and survival of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) nymphs fed on larvae of Chlosyne lacinia saundersii Doubleday & Hewitson (Lepidoptera: Nymphalidae) was studied at 25 ± 1ºC, 68 ± 10% relative humidity and 12 h photo phase. P. nigrispinus developed until the adult stage when fed on C. lacinia saundersii caterpillars. However, the survival rate of P. nigrispinus during early instars allowed this predator to complement its diet with other preys and to maintain its populations in areas with the sunflower culture.Item Predation rate of Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae with and without defense by Podisus nigrispinus (Heteroptera: Pentatomidae)(Brazilian Archives of Biology and Technology, 2008-01) Zanuncio, José Cola; Silva, Carlos Alberto Domingues da; Lima, Eraldo Rodrigues de; Pereira, Fabrício Fagundes; Ramalho, Francisco de Souza; Serrão, José EduardoThe effect of the defense of the prey Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on predation rates of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) females was studied. P. nigrispinus preyed 1.0, 1.4, 1.2, 3.8 and 3.0 and 0.4, 0.8, 1.6, 2.8 and 3.2 third instars of S. frugiperda larvae with and without defense, respectively, with the densities of one, two, four, six and eight larvae. The attack rate and the manipulation time were, respectively, 0.67 ± 0.39-h and 6.72 ± 2.88 h for P. nigrispinus females fed with larvae with defense and 2.51 ± 0.16-h and 0.51 ± 0.77 h for those without defense. The functional response of the predator P. nigrispinus varies with the defense and the density of the prey S. frugiperda, with larger consumption of prey without defense at higher densities.