Instituto de Ciências Agrárias – CRP
URI permanente desta comunidadehttps://locus.ufv.br/handle/123456789/11787
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Resultados da Pesquisa
Item Ação nematicida do eugenol em tomateiro(Pesquisa Agropecuária Tropical, 2013-07) Moreira, Lara Caroline Borges; Vieira, Bruno Sérgio; Mota Júnior, Célio Vinícius da; Lopes, Everaldo Antônio; Canedo, Ellen JúniaÓleos essenciais de várias espécies de plantas possuem propriedades nematicidas e poderiam ser utilizados no controle do nematoide das galhas. Desta forma, esta pesquisa objetivou avaliar o efeito da aplicação de concentrações de eugenol no controle de Meloidogyne incognita e Meloidogyne javanica, em casa-de-vegetação. O eugenol foi aplicado nas concentrações de 0,25 mL L-1, 0,50 mL L-1, 0,75 mL L-1 e 1,00 mL L-1, na forma de pulverização foliar ou adição ao solo. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial 4 x 2 + 1 (concentrações de eugenol x formas de aplicação + testemunha – tratamento adicional), com cinco repetições. Os números de galhas e de ovos de M. incognita e de ovos de M. javanica foram reduzidos em plantas tratadas com eugenol, em comparação com a testemunha. A adição ao solo foi o método mais eficiente de aplicação do eugenol, considerando-se a redução no número de galhas. Houve efeito significativo do aumento de concentrações de eugenol na redução do número de ovos de M. incognita. O eugenol apresenta potencial para ser utilizado no manejo do nematoide das galhas, embora novos estudos ainda sejam necessários para suportar tal expectativa.Item Soil amendment with chopped or ground dry leaves of six species of plants for the control of Meloidogyne javanica in tomato under greenhouse conditions(Ciência Rural, 2011-06) Lopes, Everaldo Antônio; Ferraz, Silamar; Ferreira, Paulo Afonso; Freitas, Leandro Grassi de; Dallemole-Giaretta, RosangelaGreenhouse experiments were conducted to evaluate the effect of soil amendment with chopped (1cm2) or ground (1mm sieve) dry leaves of assa-peixe (Vernonia polyanthes), lemon-grass (Cymbopogon citratus), eucalyptus (Eucalyptus citriodora), castor (Ricinus communis), mango (Mangifera indica) or neem (Azadirachta indica) for the control Meloidogyne javanica. Into the soil (Yellow red oxisol) of each pot were added leaves (5g kg-1 of soil) and 5,000 eggs of the nematode. After seven days, one tomato seedling "Santa Cruz Kada" was transplanted to each pot. The tomato root weight, galls and eggs/root system were determined 60 days after transplant. None of the soil amendments reduced gall or eggs, when applied as leaf pieces. However, all tested plant species reduced the gall number, when they were incorporated into the soil as powder, and maximum nematode suppression occurred in soil amended with neem leaves (61%). The amendment with ground leaves of castor, neem, eucalyptus and lemon-grass reduced the number of eggs, with maximum reduction occurring in soil amended with ground castor leaves (69%), evidencing that these organic amendments can be an alternative for M. javanica control in tomato. Further studies are required under field conditions to confirm the potential of these organic amendments on the control of M. javanica.Item Variable ITS-copy number at different developmental stages of Meloidogyne hapla and M. chitwoodi(European Journal of Plant Pathology, 2019) Lopes, Everaldo Antônio; Roberts, David Mark; Blok, Vivian CarolQuantitative polymerase chain reaction (qPCR) can be used for the accurate quantification of plant-parasitic nematodes from soil samples. Unlike the traditional Baermann funnel and centrifugal flotation techniques followed by visual enumeration, all developmental stages of plant-parasitic nematodes can be detected and quantified by using qPCR. However, little is known about the amount of DNA between different stages of plant-parasitic nematodes. Here, we show that ITS-copy number varies with developmental stage of Meloidogyne chitwoodi and M. hapla. The number of copies of ITS was lower in cell-stage eggs (51 ± 7.4 for M. hapla and 31 ± 6.0 for M. chitwoodi) than in second-stage juveniles (1345 ± 130.6 for M. hapla and 1036 ± 140.3 for M. chitwoodi) and females (19,508 ± 3367.3 for M. hapla and 9049 ± 316.7 for M. chitwoodi).Item Formulation of botanicals for the control of plant-pathogens: A review(Crop Protection, 2018-08) Borges, Darlan Ferreira; Lopes, Everaldo Antônio; Moraes, Allan Robledo Fialho; Soares, Márcio Santos; Visôtto, Liliane Evangelista; Oliveira, Cassiano Rodrigues; Valente, Vânia Maria MoreiraEssential oils and plant extracts contain a multitude of bioactive substances against fungi, bacteria and nematodes. In plant pathology research, botanicals are commonly used in their raw state. Without any type of formulation, bioactive compounds of plants can be degraded and volatilized rapidly under field conditions. Controlled-release liquid and solid formulations with plant compounds as active ingredients are common in some fields, such as medicine, pharmaceuticals, food technology and cosmetology. However, the use of controlled-release formulations is an under explored approach in plant pathology, although these technologies are interesting options for managing seed, soil-borne and post-harvest pathogens. In this review, we discuss the potential and options of formulations of botanicals against plant pathogensItem Survival of Pochonia chlamydosporia on the soil surface after different exposure intervals at ambient conditions(Revista Iberoamericana de Micología, 2017-09-27) Fernandes, Rafael Henrique; Lopes, Everaldo Antônio; Borges, Darlan Ferreira; Bontempo, Amanda Ferreira; Zanuncio, José Cola; Serrão, José EduardoExposure of the nematophagous fungus Pochonia chlamydosporia to solar radiation and elevated temperatures before being incorporated into the soil can reduce its survival and efficiency as biocontrol agent. A field experiment was carried out to assess the effect of the exposure period on the viability of P. chlamydosporia applied on the soil surface. A commercial bionematicide based on P. chlamydosporia was sprayed on soil, and soil samples were collected before and at 0, 30, 60, 90, 120, and 150 min after fungal application. Relative humidity (RH), the irradiance (IR), air temperature (AT), and soil temperature (ST) were recorded. The number of P. chlamydosporia colony forming units (CFUs) was evaluated after 20 days of incubation. P. chlamydosporia survival decreased over the time of exposure on the soil surface. Overall, the number of CFUs decreased by more than 90% at 150 min after application. Exposure to RH ≥61%, ST and AT between 25–35 ◦ C and 19–29 ◦ C, and IR between 1172 and 2126 mol of photons m −2 s −1 induced a negative exponential effect on the survival of the fungus over the time. Exposure to climatic conditions on the soil surface reduces P. chlamydosporia viability.Item Host status of progenies of yellow passion fruit to Meloidogyne incognita race 2(Crop Breeding and Applied Biotechnology, 2016-04-26) Nascimento, Roxana Stefane Mendes; Lopes, Everaldo Antônio; Santos, Carlos Eduardo Magalhães dos; Magalhães, Viviane Manuela Bernardes Silva; Cardoso, José AvelinoThe host status of eighteen progenies (CRP 01-12 to CRP 16-12, CRP 19-12 and CRP 20-12) and two cultivars (FB 200 Yellow Master and FB 300 Araguari) of yellow passion fruit to Meloidogyne incognita race 2 was evaluated in a greenhouse experiment. The height and the biomass of the plants, besides the numbers of galls, eggs and second-stage juveniles in roots and soil were assessed after 70 days of cultivation of the genotypes and tomato (control) in plastic pots with 2 kg of soil infested with 5,000 eggs of the nematode. The reproduction factor of the pathogen (RF = Final population/Initial population) was calculated for classifying the genotypes as immune (RF = 0), resistant (RF < 1) or susceptible (RF ≥ 1). The cultivar FB 200 is resistant to M. incognita race 2 and can be cultivated in areas infested with this nematode.