Artigos
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11846
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Item Can stable isotope mass spectrometry replace radiolabelled approaches in metabolic studies?(Plant Science, 2016-05-14) Silva, Willian Batista; Daloso, Danilo M.; Fernie, Alisdair R.; Nunes-Nesi, Adriano; Araújo, Wagner L.Metabolic pathways and the key regulatory points thereof can be deduced using isotopically labelled substrates. One prerequisite is the accurate measurement of the labeling pattern of targeted metabolites. The subsequent estimation of metabolic fluxes following incubation in radiolabelled substrates has been extensively used. Radiolabelling is a sensitive approach and allows determination of total label uptake since the total radiolabel content is easy to detect. However, the incubation of cells, tissues or the whole plant in a stable isotope enriched environment and the use of either mass spectrometry or nuclear magnetic resonance techniques to determine label incorporation within specific metabolites offers the possibility to readily obtain metabolic information with higher resolution. It additionally also offers an important complement to other post-genomic strategies such as metabolite profiling providing insights into the regulation of the metabolic network and thus allowing a more thorough description of plant cellular function. Thus, although safety concerns mean that stable isotope feeding is generally preferred, the techniques are in truth highly complementary and application of both approaches in tandem currently probably provides the best route towards a comprehensive understanding of plant cellular metabolism.Item Natural genetic variation for morphological and molecular determinants of plant growth and yield(Journal of Experimental Botany, 2016-03-24) Nunes-Nesi, Adriano; Nascimento, Vitor de Laia; Silva, Franklin Magnum de Oliveira; Zsögön, Agustin; Araújo, Wagner L.; Sulpice, RonanThe rates of increase in yield of the main commercial crops have been steadily falling in many areas worldwide. This generates concerns because there is a growing demand for plant biomass due to the increasing population. Plant yield should thus be improved in the context of climate change and decreasing natural resources. It is a major challenge which could be tackled by improving and/or altering light-use efficiency, CO2 uptake and fixation, primary metabolism, plant architecture and leaf morphology, and developmental plant processes. In this review, we discuss some of the traits which could lead to yield increase, with a focus on how natural genetic variation could be harnessed. Moreover, we provide insights for advancing our understanding of the molecular aspects governing plant growth and yield, and propose future avenues for improvement of crop yield. We also suggest that knowledge accumulated over the last decade in the field of molecular physiology should be integrated into new ideotypes.Item Exploring natural variation of photosynthetic, primary metabolism and growth parameters in a large panel of Capsicum chinense accessions(Planta, 2015-05-26) Rosado-Souza, Laise; Scossa, Federico; Chaves, Izabel S.; Kleessen, Sabrina; Salvador, Luiz F. D.; Milagre, Jocimar C.; Finger, Fernando; Bhering, Leonardo L.; Sulpice, Ronan; Araújo, Wagner L.; Nikoloski, Zoran; Fernie, Alisdair R.; Nunes-Nesi, AdrianoDiversity of accessions within the same species provides an alternative method to identify physiological and metabolic traits that have large effects on growth regulation, biomass and fruit production. Here, we investigated physiological and metabolic traits as well as parameters related to plant growth and fruit production of 49 phenotypically diverse pepper accessions of Capsicum chinense grown ex situ under controlled conditions. Although single-trait analysis identified up to seven distinct groups of accessions, working with the whole data set by multivariate analyses allowed the separation of the 49 accessions in three clusters. Using all 23 measured parameters and data from the geographic origin for these accessions, positive correlations between the combined phenotypes and geographic origin were observed, supporting a robust pattern of isolation-by-distance. In addition, we found that fruit set was positively correlated with photosynthesis-related parameters, which, however, do not explain alone the differences in accession susceptibility to fruit abortion. Our results demonstrated that, although the accessions belong to the same species, they exhibit considerable natural intraspecific variation with respect to physiological and metabolic parameters, presenting diverse adaptation mechanisms and being a highly interesting source of information for plant breeders. This study also represents the first study combining photosynthetic, primary metabolism and growth parameters for Capsicum to date.Item Impaired malate and fumarate accumulation due the mutation of tonoplast dicarboxylate transporter(Plant Physiology, 2017-09-20) Medeiros, David B.; Barros, Kallyne; Barros, Jessica AS; Omena-Garcia, Rebeca P; Detmann, Kelly C; Silva, Willian Batista; DaMatta, Fabio; Nunes-Nesi, Adriano; Araújo, Wagner L.; Arrivault, Stéphanie; Sanglard, Lilian Vincis Pereira; Daloso, Danilo M.; Fernie, Alisdair R.Malate is a central metabolite involved in a multiplicity of plant metabolic pathways, being associated with mitochondrial metabolism and playing significant roles n stomatal movements. Vacuolar malate transport has been characterized at the molecular level and is performed by at least one carrier protein and two channels in Arabidopsis vacuoles. The absence of the Arabidopsis thaliana tonoplast Dicarboxylate Transporter (tDT) in tdt knockout mutant was previously associated with an impaired accumulation of malate and fumarate in leaves. Here, we investigated the consequences of this lower accumulation on stomatal behaviour and photosynthetic capacity as well as ts putative metabolic impacts. Neither the stomatal conductance (g s ) nor the kinetic responses to dark, light or high CO 2 were highly affected in tdt plants. In addition, we did not observe any impact on stomatal aperture following incubation with either bscisic acid (ABA), malate or citrate. Further, an effect on photosynthetic capacity was not observed in the mutant lines. However, leaf mitochondrial metabolism was affected in the tdt plants. Levels of the intermediates of the tricarboxylic acid cycle were altered and increases in both light and dark respiration were observed. We conclude that manipulation of the tonoplastic organic acid transporter impacted mitochondrial metabolism, while the overall stomatal and photosynthetic capacity were unaffected.