Artigos
URI permanente para esta coleçãohttps://locus.ufv.br/handle/123456789/11845
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Item Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil(Hoehnea, 2018-07) Guimarães-Cestaro, Lubiane; Luz, Cynthia Fernandes Pinto da; Serrão, José Eduardo; Message, Dejair; Martins, Marta Fonseca; Alves, Maria Luisa Teles Marques Florêncio; Seijo-Coello, María Carmen; Teixeira, Érica WeinsteinRoyal jelly may contain pollen grains and their presence can be used to determine the phytogeographical origin of the product. This study analyzed the phytogeographical origin of commercial royal jelly samples from São Paulo State, tested as part of the Brazilian Federal Inspection System (SIF), found to be contaminated with spores of the bacterium Paenibacillus larvae, that causes the American Foulbrood Disease. The pollen grains of Castanea had the highest total percentage, with lower percentages of Cirsium/Carduus, Cistus, Parthenocissus, Prunus, Quercus, Robinia, Scrophulariaceae, Taraxacum, Tilia, among others. This pollen spectrum is incompatible with royal jelly samples produced in Brazil. The pollen spectrum resembled that of an imported product, compatible with the Northern Hemisphere origin. Brazilian legislation does not require the phytogeographic origin of imported bee products to be analyzed by palynological procedures, but it is mandatory to have a certificate issued by the country of origin attesting the absence of pathogens, monitored with the objective of preventing the exotic diseases from entering Brazil. Palynology, therefore, proved to be fundamental in detecting imports of this contaminated batches.Item Spores of Paenibacillus larvae, Ascosphaera apis, Nosema ceranae and Nosema apis in bee products supervised by the Brazilian Federal Inspection Service(Revista Brasileira de Entomologia, 2018-07) Guimarães-Cestaro, Lubiane; Teixeira, Érica Weinstein; Alves, Maria Luisa Teles Marques Florêncio; Message, Dejair; Martins, Marta Fonseca; Luz, Cynthia Fernandes Pinto da; Serrão, José EduardoDue to their ecological and economic importance, honey bees have attracted much scientific attention, which has intensified due to the recent population decline of these insects in the several parts of the world. Among the factors related to these patterns, infection by pathogens are the most relevant, mainly because of the easy dissemination of these microorganisms. Although no zoonotic diseases are associated with these insects, the presence of infectious agents in bee products should still be considered because they play a role as disease dispersers, increasing the risk to animal health. Because of the possibility of dispersion of pathogens via bee products, this work aimed to identify the presence of spores of the pathogens Paenibacillus larvae, Ascosphaera apis and Nosema spp. in samples of honey, pollen and royal jelly that are registered with Brazil's Federal Inspection Service (S.I.F.) and commercially available in the state of São Paulo. Of the 41 samples of bee products analyzed, only one showed no contamination by any of these pathogens. N. ceranae and P. larvae had the highest prevalence considering all the samples analyzed (present in 87.80% and 85.37% of the total, respectively), with N. apis present in 26.83% and A. apis present in 73.17% of the samples. These results provide support for the formulation of government regulations for sanitary control of exotic diseases by preventing dispersion of pathogens, including through illegal importation, since local and international trade and the transfer of colonies between regions play important roles in the dispersion of these microorganisms.Item Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome(Genetics and Molecular Biology, 2013) Sousa, Cristina Soares de; Serrão, José Eduardo; Bonetti, Ana Maria; Amaral, Isabel Marques Rodrigues; Kerr, Warwick Estevam; Maranhão, Andréa Queiroz; Ueira-Vieira, CarlosThe insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees.Item Post-embryonic development of intramandibular glands of Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae) workers(Papéis Avulsos de Zoologia, 2017) Oliveira, André Henrique de; Lisboa, Luciane Cristina de Oliveira; Serrão, José Eduardo; Zanuncio, José Cola; Souza, Edmilson Amaral deExocrine glands play important role in social organization of insects, such as caste and inter-caste differentiation. Due their functional and structural plasticity, morphological studies on such glands contribute to better understanding the biology of social bees. Therefore, the aim of the study was to characterize the sequence of the post-embryonic development of intramandibular glands of Friesella schrottkyi (Friese, 1900) (Hymenoptera: Apidae) workers using histological and histochemical analyses. The mandibles of pupae at different developmental stages and newly emerged adults were analyzed. The intramandibular glands of F. schrottkyi presented two types: class I glands, in the mandible epidermis and class III glands, inside the mandible cavity that open onto external surface. The intramandibular glands of F. schrottkyi developed during the transition from the prepupae to the white-eyed pupae, as shown by the morphological changes. Black-eyed pupae of F. schrottkyi presented fully developed intramandibular glands.Item Pukuakanga salignea, gênero novo e espécie nova de Dictyopharidae (Hemiptera: Auchenorrhyncha: Fulgoromorpha) do Sudeste do Brasil(Biota Neotropica, 2010-10) Baptista, Marcelo da Silva; Serrão, José Eduardo; Da-Silva, Elidiomar RibeiroPukuakanga gen.nov. (Hemiptera: Dyctiopharidae) é erigido com base em exemplares adultos de uma espécie nova, Pukuakanga salignea sp.nov., provenientes de diferentes municípios do Estado de Minas Gerais, Brasil. O gênero pode ser determinado por uma combinação de características como: cabeça com o comprimento 4,8× a largura da base; ápice da projeção da cabeça apresentando a união das carenas marginais da fronte e do vértice lateralmente a projeção; ausência de carena na tégula; margem anteromediana do pronoto reta; três fileiras de células na membrana da asa anterior; edeago com a conjuntiva bifurcada formando dois pares de tubos e o espinho do pênis apresentando um ramo lateral.Item Exposure to insecticides reduces populations of Rhynchophorus palmarum in oil palm plantations with bud rot disease(Insects, 2019-04) Martínez, Luis Carlos; Plata-Rueda, Angelica; Rodríguez-Dimaté, Francisco Andrés; Campos, Juliana Mendonça; Santos Júnior, Valdeir Celestino dos; Rolim, Gabriela Da Silva; Fernandes, Flavio Lemes; Silva, Wiane Meloni; Wilcken, Carlos Frederico; Zanuncio, José Cola; Serrão, José EduardoThe South American palm weevil (SAPW), Rhynchophorus palmarum Linnaeus (Coleoptera: Curculionidae) is the main pest of Elaeis guineensis and damages palm trees with bud rot disease in the Americas. The effects of six neurotoxic insecticides (abamectin, carbaryl, deltamethrin, fipronil, imidacloprid and spinosad) were evaluated against SAPW for toxicity, survival, reproduction, and mortality. Abamectin (LC50 = 0.33 mg mL−1 ), Carbaryl (LC50 = 0.24 mg mL−1 ), deltamethrin (LC50 = 0.17 mg mL−1 ), and fipronil (LC50 = 0.42 mg mL−1 ) were the most toxic to SAPW. Adult survival was 95% without exposure to insecticides, decreasing to 78–65% in insects treated with the LC25 and 49–35% in insects exposed to LC50 . Sublethal doses of carbaryl, fipronil and imidacloprid showed significant effect on the reproduction of this insect. Mortality of SAPW populations caused by insecticides had similar effects in the laboratory and field conditions. The results suggest that carbaryl, deltamethrin, fipronil, and imidacloprid caused significantly higher mortality as compared to the control in SAPW and may be used to control its populations in oil palm trees where bud rot appears as the key disease for SAPW attraction and infestation.Item Morphology of the mandibular gland of the ant Paraponera clavata (Hymenoptera: Paraponerinae)(Microscopy Research and Technique, 2019) Andrade, Thito Thomston; Gonçalves, Wagner Gonzaga; Serrão, José Eduardo; Martins, Luiza Carla BarbosaThe ant Paraponera clavata (Fabricius, 1775) is the only extant species of Paraponerinae and is widely distributed in Brazilian forests. Aspects of its biology are documented extensively in the literature; however, knowledge of P. clavata internal morphology, specifically of exocrine glands, is restricted to the venom apparatus. The objective of this study was to describe the mandibular gland morphology of P. clavata workers. The mandibular gland is composed of a reservoir connected to a cluster of Type III secretory cells with cytoplasm rich in mitochondria and lipid droplets, similar to that of other ants. Notably, the glandular secretion is rich in protein and has a solid aspect. This is the first morphological description of the mandibular gland of P. clavata.Item Histochemistry, immunohistochemistry and cytochemistry of the anterior midgut region of the stingless bee Melipona quadrifasciata and honey bee Apis mellifera (Hymenoptera: Apidae)(Micron, 2018-10) Carneiro, Lenise Silva; Gonçalves, Wagner Gonzaga; Fernandes, Kenner Morais; Zanuncio, José Cola; Serrão, José Eduardo; Teixeira, Stéphanie Asséf Millen ValenteThe anterior midgut region of stingless bees is anatomically differentiated with tall and narrow cells, whereas in other social and solitary bees this anatomical gut region is lacking. The objective of the present study was to describe the histochemistry, immunohistochemistry and cytochemistry of the anterior midgut region of the stingless bee Melipona quadrifasciata in comparison with the honey bee Apis mellifera. The anterior midgut region of both species was evaluated for identification of the enzymes β- galactosidase, glucose-6-phosphatase, acid phosphatase, and alkaline phosphatase, the membrane transporter aquaporin, the hormone FMRF-amide, and lysosomes. Histology of the anterior midgut region showed that this region in M. quadrifasciata workers did not present external folds of the wall, whereas the following midgut wall presented many. In A. mellifera, folds in the midgut wall occur starting from the fore- midgut transition region. Despite these morphological differences, the tests evaluated were similar in both species. β-galactosidase was not found in the anterior midgut cells. Glucose-6-phosphatase and acid phosphatase occurred in the apical region of the gut epithelium. Alkaline phosphatase occurred in vesicles in apical cytoplasm and in the basal plasma membrane infoldings of the epithelial cells. Aquaporin was found in the basal region of the midgut epithelium and in the associated visceral muscles. FMRF-amide was found only in nerve endings in the anterior midgut region. All cells in the anterior midgut region were rich in lysosomes. These results suggest that in both bee species, although they have anatomically different anterior midgut regions, these regions present high metabolic activity and function in cellular homeostasis, lipid absorption and are under neurohormone control.Item Aedes aegypti larvae treated with spinosad produce adults with damaged midgut and reduced fecundity(Chemosphere, 2019-04) Fernandes, Kenner Morais; Tomé, Hudson Vaner Ventura; Miranda, Franciane Rosa; Gonçalves, Wagner Gonzaga; Pascini, Tales Vicari; Serrão, José Eduardo; Martins, Gustavo Ferreirapt-BRThe mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity and mortality. The fight against these pathogens is mainly based on the control of the insect vector with the use of insecticides. Among insecticides, spinosad bioinsecticide is efficient against A. aegypti larvae and may be an alternative for vector control. Here, we investigate the sublethal effects of spinosad during midgut metamorphosis of A. aegypti females and its cumulative effects on blood acquisition capacity and fecundity in adults. We studied the midgut because it is an important model organ directly related to blood acquisition and digestion. Treatment of larvae with spinosad induced oxidative stress, apoptosis, and damage to the midgut cells at all stages of development and in adults. There was a reduction in the number of proliferating cells and the number of enteroendocrine cells in treated individuals. In addition, damage caused by spinosad led to a reduction in oviposition and egg viability of A. aegypti females. Finally, the exposure of mosquito larvae to sublethal concentrations of spinosad interfered with the development of the midgut, arresting the blood digestion and reproduction of adult females with blood digestion and reproduction difficulties.Item Histological evidence of a glandular tubercle at the abdominal tergite in three species of katydids (Orthoptera: Tettigoniidae)(Zoologischer Anzeiger, 2018-05) Serrão, José Eduardo; Salazar, Karen; Chamorro-Rengifo, JulianaThree species of Hyperophora Brunner von Wattenwyl have a tubercle on the second abdominal tergite with different external appearance among species. We predicted that the tubercles are a glandular structure for their morphology and anatomical position. In H. major Brunner von Wattenwyl and H. brasiliensis Brunner von Wattenwyl the tubercle is low and broad, whereas in H. minor Brunner von Wattenwyl it is high. In all species the tubercle does not have an external opening, but it has many chemosensory sensilla, evidenced by long setae with an internal cavity with a membrane-like content. The cuticular surface of the tubercle has many pores with ∼1–2 μm in diameter scattered among the sensilla. On the inner surface of the tubercular region occur epidermal gland cells, evidenced by a mass of large globular cells with an irregular and well-developed nucleus with predominance of decondensed chromatin and a large nucleolus. The cytoplasm of these cells is rich in small clear vacuoles, and has an end apparatus, a feature of epidermal gland cells of class III. Each gland cell has a conducting canal that opens into a cuticular pore. These results support the hypothesis that the tubercles are indeed of glandular nature.