Navegando por Autor "Serrao, Jose Eduardo"
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Item FMRFamide-like midgut endocrine cells during the metamorphosis in Melipona quadrifasciata anthidioides (Hymenoptera, Apidae)(Micron, 2001-12-05) Neves, Clóvis Andrade; Bhering, Leonardo Lopes; Serrao, Jose Eduardo; Gitirana, Lycia BritoThe FMRFamide, gastrin and cholecystokinin (CCK) occurrence in endocrine cells of insects has been described by several authors, although their functions are still not well defined for this group of animals. In the present study, the occurrence of endocrine cells producing FMRFamide, gastrin 1 and CCK-8 in the midgut (ventriculus) of Melipona quadrifasciata anthidioides (Hymenoptera, Apidae), before, during and after the metamorphosis, were investigated by means of pre-embedding immunofluorescence techniques. FMRFamide reactivity was found in the endocrine cells as well as in the nervous fibers and neurons of the intestine of these bees. ‘Open’ and ‘closed’ types of FMRFamide-like cells were observed in last instar larvae. In the black eyed pupae the producing cells of FMRFamide seemed to be immature, and, in the workers, where the FMRFamide producing cells were more abundant, the production of this substance seemed to occur only in the open cells. Reactivity of the nervous fibers and neurons were observed, during the prepupae, white eyed pupae, and pink eyed pupae. The same did not occur with the midgut endocrine cells. There were no immunoreactivity observations for gastrin 1 and for CCK-8. The FMRFamide-like cells were present in the midgut of these insects during or close to the period that they were eating, which indicates that the FMRFamide may be involved in the control of the digestive process.Item Proventriculus of three nemobiinae crickets (Orthoptera: Grylloidea: Trigonidiidae)(Journal of Orthoptera Research., 2009) Serrao, Jose Eduardo; Sperber, Carlos Frankl; Szinwelski, Neucir; Rodrigues, Moreno S.; Pereira, Marcelo RibeiroThe morphology of the proventriculus may supply useful taxonomic characters for some species of crickets. This study evaluated if the proventriculus can be used to distinguish three species of Nemobiinae crickets: Phoremia sp. n., Zucchiella sp. n., and Amanayara sp n. In crickets the proventriculus presents six lobes, each one bearing eight appendices along its longitudinal axis. In Phoremia sp. n., the central portion of the first appendix has a tooth like a spear, with two denticles; the central portion of the second appendix presents one plate formed by five denticles, whereas in Zucchiella sp. n., the apex of the first appendix is LI-shaped with denticles absent, and the second appendices differ from those of Phoremia sp. n. by the number of denticles. In Amanayara sp. n. the central portion of the first and second appendices form a long and sharpened tooth, the denticles are lacking. In the other proventricular appendices there was variation in the number of denticles and in their shape. These results indicate that the morphology of the proventriculus can provide auxiliary characters for taxonomy of Nemobiinae, especially useful for this subfamily, since for many species the external morphology is insufficient for characterization of genera and species.