Navegando por Autor "Duarte, M. S."

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Bovine mature adipocytes readily return to a proliferative state
(Tissue and Cell, 2012-12) Duarte, M. S.; Wei, S.; Du, M.; Paulino, P. V. R.; Jiang, Z.; Albrecht, E.; Fernyhough-Culver, M.; Zan, L.; Hausman, G. J.; Dodson, M. V.
The dynamics of human and animal adipogenesis has been defined using several traditional cell systems including stromal vascular cells and adipocyte-related cell lines. But a relatively new cell system using progeny cells stemming from the dedifferentiation of purified cultures of mature adipocytes may be used for studying the development and biology of adipocytes. In this research, we show that isolated (and purified) mature adipocytes derived from Wagyu cattle dedifferentiate into progeny cells, and that these spindle-shaped, proliferative-competent daughter cells possess ability to proliferate. We outline the optimum cell culture system and offer precautionary thoughts for effective mature adipocyte culture. Collectively, this represents a novel cell model which may provide new insights into cell development, physiology and use as a model for animal production/composition, tissue engineering and disease treatment.
Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat
(Meat Science, 2014-01) Lage, J. F.; Paulino, P. V. R.; Pereira, L. G. R.; Valadares Filho, S. C.; Oliveira, A. S.; Duarte, M. S.; Souza, N. K. P.; Lima, J. C. M.
Thirty non-castrated male lambs with 20 ± 2.3 kg average body weight (BW) were randomly assigned to five treatments consisted of different dietary concentrations of crude glycerin (CG; 0, 3, 6, 9 and 12% on DM basis) to evaluate the effects on performance, carcass and meat quality traits. A quadratic effect was observed for performance (P = 0.04), final BW (P b 0.01) and hot carcass weight (P b 0.01). No effects of CG were observed (P > 0.05) on carcass pH neither on shear-force, cooking loss and ether extract content in longissimus. The inclusion of CG tended to reduce the Zn content in meat (P = 0.09). The data suggests that CG (36.2% of glycerol and 46.5% of crude fat) may be used in diets of finishing lambs with concentrations up to 3% without negative effects on performance and main carcass traits. Moreover, inclusion of CG seems to not affect quality and safety of meat for human consumption.
Differences in skeletal muscle proteolysis in Nellore and Angus cattle might be driven by Calpastatin activity and not the abundance of Calpain/Calpastatin
(The Journal of Agricultural Science, 2017-12) Martins, T. S.; Sanglard, L. M. P.; Silva, W.; Chizzotti, M. L.; Ladeira, M. M.; Serão, N. V. L.; Paulino, P. V. R.; Duarte, M. S.
The present study aimed to explore the molecular factors underlying differences in Calpain/Calpastatin proteolytic system in Nellore and Angus cattle. Longissimus muscle samples were collected in Nellore (n = 6; body weight (BW) = 373 ± 37·3 kg) and Angus (n = 6; BW = 383 ± 23·9 kg) cattle at slaughter for analysis of gene and protein expression, and Calpastatin enzyme activity. Additionally, the myofibrillar fragmentation index was used to quantify the extension of proteolysis in longissimus muscle samples. A greater myofibrillar fragmentation was observed in skeletal muscle of Angus compared with Nellore cattle. Conversely, no differences were found between breeds for mRNA expression of Calpain 1 (CAPN1) and Calpastatin (CAST). Similarly, no differences were observed for the abundance of Calpain and Calpastatin proteins between skeletal muscles of Nellore and Angus cattle. Despite the lack of differences in mRNA and protein abundance, a greater activity of Calpastatin was observed in skeletal muscle of Nellore compared with Angus cattle. These data indicate that the greater proteolysis in skeletal muscle of Angus compared with Nellore cattle is mainly driven by a greater Calpastatin activity rather than Calpain or Calpastatin mRNA and protein expression.
Effects of maternal nutrition on development of gastrointestinal tract of bovine fetus at different stages of gestation
(Livestock Science, 2013-05) Duarte, M. S.; Gionbelli, M. P.; Paulino, P. V. R.; Serão, N. V. L.; Martins, T. S.; Tótaro, P. I. S.; Neves, C. A.; Valadares Filho, S. C.; Dodson, M. V.; Zhu, M.; Du, M.
This study was developed aiming to evaluate the effects of maternal feed-restriction ond evelopment of gastrointestinal tract (GIT) of bovine fetus at different gestational stages. Feed-restricted cows were fed 1.2 times the maintenance level while the control group was fed ad libitum. Pregnant cows were slaughtered at 136, 189, 239, and 269 days of gestation and gastrointestinal tracts of the fetuses were evaluated. No effects of maternal nutrition on body weight (P 1⁄4 0.17) and body length (P 1⁄40.13) of the fetuses were observed. No major effects of feed restriction on GIT mass of the fetuses were observed (P 1⁄4 0.51). However, the weight of small intestine per unit of body weight was 11.24% greater (P 1⁄40.04) in fetuses from restricted dams. Additionally, the length of small intestine and its villi were 12.93% and 16.44% respectively greater (P o .001) in fetuses from restricted dams compared to those from non-restricted dams. These data indicates that maternal feed-restriction does not affect the development of most of fetal gastrointestinal parts besides small intestine which in turn increases its surface area as a response of maternal feed restriction.
Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers
(Journal of Dairy Science, 2016-12) Weller, M. M. D. C. A.; Albino, Ronan L.; Marcondes, M. I.; Silva, W.; Daniels, K. M.; Campos, M. M.; Duarte, M. S.; Mescouto, M. L.; Silva, F. F.; Guimarães, S. E. F.
This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n = 6 per treatment) designed to sustain an average daily gain of 0.0 kg/d (maintenance, MA), 0.5 kg/d (low gain, LG), or 1.0 kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated.
Effects of pregnancy and feeding level on carcass and meat quality traits of Nellore cows
(Meat Science, 2013-05) Duarte, M. S.; Gionbelli, M. P.; Paulino, P. V. R.; Serão, N. V. L.; Silva, L. H. P.; Mezzomo, R.; Dodson, M. V.; Du, M.; Busboom, J. R.; Guimarães, S. E. F.; Valadares Filho, S. C.
Carcass and meat quality traits of 16 pregnant and 5 non-pregnant cows fed at 1.2 times maintenance and 16 pregnant and 6 non-pregnant fed ad libitum were evaluated. Pregnancy did not affect final body weight (FBW; P = 0.0923), cold carcass yield (CCY; P = 0.0513), longissimus muscle area (LMA; P = 0.8260), rib fat thickness (RFT; P = 0.1873) and shear force (WBSF; P = 0.9707). A lower FBW (P = 0.0028), LMA (P = 0.0048) and RFT (P = 0.0001) were observed in feed restricted cows. However, no differences were found for CCY (P = 0.7243) and WBSF (P = 0.0759) among feeding level groups. These data suggests that carcass and meat quality traits are not affected by pregnancy status in Nellore cows. Moreover, although cows experiencing feed restriction did have reduced deposition of subcutaneous fat and lean tissue, there were no major impacts on meat quality traits.
Foetal development of skeletal muscle in bovines as a function of maternal nutrition, foetal sex and gestational age
(Journal of Animal Physiology and Animal Nutrition, 2017-10-10) Gionbelli, T. R. S.; Veloso, C. M.; Rotta, P. P.; Valadares Filho, S. C.; Carvalho, B. C.; Marcondes, M. I.; Cunha, C. S.; Novaes, M. A. S.; Prezotto, L. D.; Duarte, M. S.; Gionbelli, M. P.
To determine the effects of maternal nutrition on modifications of foetal development of the skeletal muscle and possible increase in the potential of skeletal muscle growth in cattle, gestating cows were either fed 190% NRC recommendations (overnourished; ON) or 100% NRC recommendation (control; CO). Interaction between maternal nutrition (MN) and the foetal sex (FS) was also investigated. Foetuses were necropsied at four different time points throughout gestation (139, 199, 241 and 268 days of gestation) to assess the mRNA expression of myogenic, adipogenic and fibrogenic markers in skeletal muscle. Phenotypic indicators of the development of skeletal muscle fibres, intramuscular lipogenesis and collagen development were also evaluated. Modifications in mRNA expression of skeletal muscle of foetuses were observed in function of MN and FS despite the lack of effect of MN and FS on foetal weight at necropsy. Maternal ON increased the mRNA expression of the myogenic marker Cadherin-associated protein, beta 1 (CTNNB1) and adipogenic markers Peroxissome proliferator-activated receptor gamma (PPARG) and Zinc finger protein 423 (ZNF423) at midgestation. However, no differences on foetal skeletal muscle development were observed between treatments at late gestation indicating that a compensatory development may have occurred on CO foetuses making the effect of MN on skeletal muscle development not significant at late gestation. Moreover, our data have shown an evidence of sexual dimorphism during foetal stage with a greater skeletal muscle development in male than in female foetuses. In conclusion, providing a higher nutritional level to pregnant cows changes the trajectory of the development of skeletal muscle during midgestation, but apparently does not change the potential of post-natal growth of muscle mass of the offspring, as no differences in skeletal muscle development were observed in late gestation.
Influence of condensed tannin on intake, digestibility, and efficiency of protein utilization in beef steers fed high concentrate diet
(Livestock Science, 2011-10) Mezzomo, R.; Paulino, P. V. R.; Detmann, E.; Valadares Filho, S. C.; Paulino, M. F.; Monnerat, J. P. I. S.; Duarte, M. S.; Silva, L. H. P.; Moura, L. S.
This trial was conducted to evaluate if the effect of condensed tannin (CT) is associated with a true protein source on intake, ruminal and total digestibility, ruminal digestion rate, protein efficiency, and microbial efficiency in beef steers fed high concentrate diet (87% of DM). Four Bos indicus steers (407 ± 12 kg of BW) fitted with rumen cannula were assigned to a 4 × 4 Latin square design, arranged in a 2 × 2 factorial arrangement. Treatments consisted of either inclusion (0.4% of DM) or exclusion of condensed tannin (CT) from quebracho extract (76% of CT) with or without the use of soybean meal (SBM) as source of true protein. The level of inclusion was calculated to provide a daily intake of 1 g/10 kg of BW of CT. Intake of DM and nutrients was not affected (P > 0.10) by CT inclusion. However, there was an effect (P < 0.10) of CT inclusion on ether extract digestibility. An interaction (P < 0.10) was observed between CT and SBM on ruminal digestibility and digestion rate of crude protein (CP): when mixed with soybean meal, CT decreased the ruminal digestibility and, consequently, reduced the digestion rate of CP. Intake of CP increased (P < 0.10) with the inclusion of SBM. No differences in DM passage rate were observed (P > 0.10) among treatments. Effects of the interaction (P < 0.10) between CT and SBM were observed on flux of rumen undegradable protein (RUP), metabolizable protein (MP), and on the ratio MP:CP. In the presence of soybean meal, the addition of CT increased (P < 0.10) the flux of RUP and MP, and improved the ratio MP:CP. The yield of microbial protein on the abomasum and the microbial efficiency did not differ among treatments (P > 0.10). There was no difference (P > 0.10) on the pH, VFA, and ruminal ammonia (N-NH3) with the addition of condensed tannin. The N-NH3 increased and the ruminal pH decreased with the inclusion of soybean meal (P < 0.10). The utilization of condensed tannin as an additive in beef cattle diets with high level of concentrate and soybean meal as a source of true protein implies positive effects on crude protein utilization, decreasing digestion rate and ruminal digestibility of crude protein and consequently increasing the levels of metabolizable protein, with no changes in the ruminal fermentation parameters.
Influence of genetic type and level of concentrate in the finishing diet on carcass and meat quality traits in beef heifers
(Meat Science, 2012-03) Paulino, P. V. R.; Valadares Filho, S. C.; Duarte, M. S.; Benedeti, P. D. B.; Souza, N. K. P.; Lage, J. F.; Souza, E. J. O.; Cox, R. B.
Carcass and meat quality traits of thirty-six feedlot beef heifers from different genetic groups (GG) fed at two concentrate levels (CL) were evaluated using 12 - Nellore (NE), 12 - ½Angus x ½Nellore (AN) and 12 - ½Simmental x ½Nellore (SN) animals. Six heifers of each GG were randomly assigned into one of two treatments: concentrate at 0.8% or 1.2% of body weight (BW). Heifers fed concentrate at 0.8% of BW had greater (P < 0.05) dressing percentage. None of the proximate analysis components of the beef were affected (P > 0.05) by either CL or GG. Heifers from the AN group had higher (P < 0.05) carcass weights, 12th rib fat thickness and lower dressing percentage (P < 0.05) compared to the other groups. NE heifers had greater WBSF values (P < 0.05) than the other genetic groups. Data suggest that the concentrate level can be reduced without compromising meat quality traits.
Intestinal development of bovine foetuses during gestation is affected by foetal sex and maternal nutrition
(Journal of Animal Physiology and Animal Nutrition, 2016-08-03) Rotta, P. P.; Gionbelli, T. R. S.; Veloso, C. M.; Valadares Filho, S. C.; Carvalho, B. C.; Marcondes, M. I.; Ferreira, M. F. L.; Souza, J. V. F.; Santos, J. S. A. A.; Lacerda, L. C.; Duarte, M. S.; Gionbelli, M. P.
We aimed to evaluate the effects of maternal nutrition (MN) and foetal sex on the intestinal development of bovine foetuses throughout different days of gestation (DG). Forty-four multiparous, dry Holstein 9 Gyr cows with average initial body weight of 480 Æ 10 kg were fed the same diet of either restricted feeding at 1.15% of body weight (CO, n = 24) or fed ad libitum (overnourished, ON, n = 20). Six cows from CO group and five cows from ON group were slaughtered at 139, 199, 241 and 268 DG, and foetuses were necropsied to evaluate the intestinal development. The mass, length and density of foetal intestines were not affected by MN (p ≥ 0.260). An interaction between MN and DG was observed for the villi length of jejunum (p = 0.006) and ileum (p < 0.001). Villi length of jejunum and ileum was higher (p < 0.10) in foetuses from ON-fed cows than in foe- tuses from CO-fed cows at 139 DG. However, at 199 DG, the villi length of jejunum and ileum of foetuses from CO-fed cows was higher than in foetuses from ON-fed cows. Despite these differences, MN did not affect the villi length of jejunum and ileum at 268 DG (p > 0.10). Female foetuses had greater small intestine mass (p = 0.093), large intestine mass (p = 0.022), small intestine mass in proportion to body mass (p = 0.017) and large intestine mass in proportion to body mass (p < 0.001) than male foetuses. Female foetuses had also longer small intestine (p = 0.077) and greater small intestine density (p = 0.021) and villi length of jejunum (p = 0.001) and ileum (p = 0.010) than males. We conclude that MN affects the pathway for the development of foetal villi length throughout the gestation in bovine foetuses without changing the final villi length. Female foetuses had higher intestinal mass, density and villi length than males during the foetal phase in bovines.
Performance strategies affect mammary gland development in prepubertal heifers
(Journal of Dairy Science, 2017-10) Albino, R. L.; Sguizzato, A. L.; Daniels, K. M.; Duarte, M. S.; Lopes, M. M.; Guimarães, S. E. F.; Weller, M. M. D. C. A.; Marcondes, M. I.
In Brazil, the majority of dairy cattle are Holstein × Gyr (H×G). It is unknown whether excessive energy intake negatively affects their mammary development to the same extent as in purebred Holsteins. We hypothesized that mammary development of H×G heifers can be affected by dietary energy supply. We evaluated the effect of different average daily gains (ADG) achieved by feeding different amounts of a standard diet during the growing period on biometric measurements, development of mammary parenchyma (PAR) and mammary fat pad (MFP), and blood hormones. At the outset of this 84-d experiment, H×G heifers (n = 18) weighed 102.2 ± 3.4 kg and were 3 to 4 mo of age. Heifers were randomly assigned to 1 of 3 ADG programs using a completely randomized design. Treatments were high gain (HG; n = 6), where heifers were fed to gain 1 kg/d; low gain (LG; n = 6), where heifers were fed to gain 0.5 kg/d; and maintenance (MA; n = 6), where heifers were fed to gain a minimal amount of weight per day. Heifers were fed varying amounts of a single TMR to support desired BW gains. Over the 84 d, periodic biometric and blood hormone measurements were obtained. On d 84, all heifers were slaughtered and carcass and mammary samples were collected. At the end, HG heifers weighed the most (181 ± 7.5 kg), followed by LG (146 ± 7.5 kg) and MA (107 ± 7.5 kg) heifers. The ADG were near expected values and averaged 0.907, 0.500, and 0.105 ± 0.03 kg/d for HG, LG, and MA, respectively. In addition, body lengths, heart girths, and withers heights were affected by dietary treatment, with MA heifers generally being the smallest and HG heifers generally being the largest. Body condition scores differed by treatment and were highest in HG and lowest in MA heifers; in vivo subcutaneous fat thickness measurement and direct analysis of carcass composition supported this. The HG heifers had the heaviest MFP, followed by LG and then MA heifers. Amount of PAR was highest in LG heifers and was the same for HG and MA heifers. The percentage of udder mass occupied by PAR was lowest in HG heifers, differing from LG and MA heifers. Composition of MFP was not evaluated. Regarding PAR composition, no differences in ash or DM were found. On the other hand, CP concentration of PAR for HG heifers was lower than that for LG heifers, which was lower than that for MA heifers. Regarding the fat content, HG treatment was higher than LG and MA treatment, which did not differ from each other. In PAR, differences in relative abundance of genes related to both stimulation and inhibition of mammary growth were observed to depend on dietary treatment, sampling day, or both. The same can be said for most of the blood hormones that were measured in this experiment. In this experiment, high ADG achieved by feeding different amounts of a standard diet during the growing period negatively affected mammary development.
Review: Animal model and the current understanding of molecule dynamics of adipogenesis
(Animal, 2016-06) Campos, C. F.; Duarte, M. S.; Guimarães, S. E. F.; Verardo, L. L.; Wei, S.; Du, M.; Jiang, Z.; Bergen, W. G.; Hausman, G. J.; Fernyhough-Culver, M.; Albrecht, E.; Dodson, M. V.
Among several potential animal models that can be used for adipogenic studies, Wagyu cattle is the one that presents unique molecular mechanisms underlying the deposit of substantial amounts of intramuscular fat. As such, this review is focused on current knowledge of such mechanisms related to adipose tissue deposition using Wagyu cattle as model. So abundant is the lipid accumulation in the skeletal muscles of these animals that in many cases, the muscle cross-sectional area appears more white (adipose tissue) than red (muscle fibers). This enhanced marbling accumulation is morphologically similar to that seen in numerous skeletal muscle dysfunctions, disease states and myopathies; this might indicate cross-similar mechanisms between such dysfunctions and fat deposition in Wagyu breed. Animal models can be used not only for a better understanding of fat deposition in livestock, but also as models to an increased comprehension on molecular mechanisms behind human conditions. This revision underlies some of the complex molecular processes of fat deposition in animals.
Soybean meal replaced by slow release urea in finishing diets for beef cattle
(Livestock Science, 2014-07) Benedet, Pedro Del Bianco; Paulino, Pedro Veiga Rodrigues; Marcondes, Marcos I.; Valadares Filho, Sebastião de Campos; Martins, Taiane S.; Lisboa, E. F.; Silva, Luiz H. P.; Teixeira, César Roberto Viana; Duarte, M. S.
Eight crossbred steers (average body weight of 418 kg) fitted with ruminal and abomasal cannula were used to evaluate the effects of replacing soybean meal (SBM) with slow-release urea (SRU) in beef cattle diets containing two concentrate levels. The experimental design included two 4×4 Latin squares, which were run simultaneously. Each Latin square received one level of concentrate [400 or 800 g/kg on a dry matter (DM) basis]. Within each Latin square, the four replacement levels of soybean meal protein with slow-release urea were applied to the animals (0%, 33%, 66% and 100% of substitution on N basis). The DM intake as well as organic matter (OM) intake and crude protein (CP) intake decreased linearly (P<0.05) as SBM was replaced with SRU. Ruminal digestibility coefficient of OM tended to be greater (P=0.074) for the 40 % concentrate diet. DM and OM passage rate (kp) were greater (P<0.05) on the 80% concentrate diet. A cubic effect (P<0.10) of SBM replacement with SRU on ruminal ammonia (NH3–N) concentration in relation to time was detected. A quadratic effect on pH was observed (P<0.10) when replacing SBM with SRU. Nitrogen intake, nitrogen excreted in the feces, nitrogen balance and efficiency of nitrogen use decreased linearly (P<0.10) as SRU increased in the diet, whereas the total nitrogen excreted in urine increased linearly (P=0.007). The production of microbial nitrogen and microbial efficiency were not affected by the experimental treatments (P>0.10). A lower intake of DM, OM, and CP was observed when cattle were fed SRU compared to SBM. However, the use of SRU did not change the digestibility and digestion rate (kd) and kp of DM, OM, CP and neutral detergent fiber corrected for ash and protein (NDFap). In summary, SRU provides higher concentrations of NH3–N throughout a day than SBM in cattle fed low concentrate diets.
Supplementation of grazing beef cows during gestation as a strategy to improve skeletal muscle development of the offspring
(Animal, 2017-12) Marquez, D. C.; Paulino, M. F.; Rennó, L. N.; Villadiego, F. C.; Ortega, R. M.; Moreno, D. S.; Martins, L. S.; Almeida, D. M. de; Gionbelli, M. P.; Manso, M. R.; Melo, L. P.; Moura, F. H.; Duarte, M. S.
The appropriate supply of nutrients in pregnant cows has been associated with the optimal development of foetal tissues, performance of their progeny and their meat quality. The aim of this study was to evaluate supplementation effects of grazing cows in different stages of gestation on skeletal muscle development and performance of the progeny. Thereby, 27 Nellore cows were divided into three groups (n=9 for each group) and their progeny as follows: UNS, unsupplemented during gestation; MID, supplemented from 30 to 180 days of gestation; LATE, supplemented from 181 to 281 days of gestation. The percentage composition of the supplement provided for the matrices was the following: ground corn (26.25%), wheat bran (26.25%) and soya bean meal (47.5%). The supplement was formulated to contain 30% CP. Supplemented matrices received 150 kg of supplement (1 and 1.5 kg/day for cows in the MID and LATE groups, respectively). After birth, a biopsy was performed to obtain samples of skeletal muscle tissue from calves to determine number and size of muscle fibres and for messenger RNA (mRNA) expression analysis. The percentage composition of the supplement provided for the progeny was the following: ground corn grain (30%), wheat bran (30%), soya bean meal (35%) and molasses (5%). The supplement was formulated to contain 25% CP and offered in an amount of 6 g/kg BW. Performance of the progeny was monitored throughout the suckling period. Means were submitted to ANOVA and regression, and UNS, MID and LATE periods of supplementation were compared. Differences were considered at P<0.10. Birth weight, average daily gain and weaning weight of the offspring did not differ among treatments (P>0.10). Similarly, no differences were observed between calves for nutrient intake (P>0.10). However, greater subcutaneous fat thickness (P=0.006) was observed in the calves of LATE group. The ribeye area (P=0.077) was greater in calves born from supplemented compared with UNS cows. The supplementation of pregnant cows did not affect the muscle fibre size of their progeny (P=0.208). On the other hand, calves born from dams supplemented at mid-gestation had greater muscle fibre number (P=0.093) compared with calves from UNS group. Greater mRNA expression of peroxysome proliferator-activated receptor α (P=0.073) and fibroblast growth factor 2 (P=0.003) was observed in the calves born from MID cows. Although strategic supplementation did not affect the BW of offspring, it did cause changes in carcass traits, number of myofibres, and mRNA expression of a muscle hypertrophy and lipid oxidation markers in skeletal muscle of the offspring.
Technical Note: A comparison among adipogenic induction protocols for dedifferentiated fat (DFAT) cells obtained from subcutaneous fat of pigs
(Livestock Science, 2017-05) Bueno, R.; Campos, C. F. de; Veroneze, R.; Silva, W.; Sanglard, L. M. P.; Alcantara, L.; Serão, N. V. L.; Hausman, G. J.; Dodson, M. V.; Duarte, M. S.; Guimarães, S. E. F.
In the current study we have performed two experiments to evaluate the effects of adipogenic induction media on dedifferentiated fat (DFAT) cells redifferentiation into mature adipocytes. In experiment 1, we aimed to evaluate whether it is necessary to use insulin in the induction media to allow DFAT cells differentiation into mature adipocytes by establishing two experimental treatments where insulin was either withdrawn from the culture medium, after 72 h of a normal induction period (Treatment 1: Insulin-) or kept in culture media (Treatment 2: Insulin+) for 16 d. In experiment 2, we aimed to evaluate if the lack of 3-isobutyl-1-methylxanthine (IBMX) in the induction medium would affect the differentiation of DFAT cells into mature adipocytes. For that, DFAT cells were induced to differentiate into lipid assimilating adipocytes using an induction medium containing IBMX (Treatment 1: IBMX+) or without IBMX (Treatment 2: IBMX-) during the first 72 h of induction. In both experiments we have evaluated the mRNA expression of lipid metabolism markers and cell morphology through Oil-Red staining as indicators of differentiation of DFAT cells into lipid-assimilating cells. The results of Experiment 1 revealed no differences in mRNA expression for any of the lipid metabolism markers with exception of GLUT4 (P=0.02), which was greater in Insulin- compared to Insulin+ treatment. Similarly, no differences were observed for mRNA expression of adipogenic markers between IBMX+ and IBMX- treatments with exception of FABP4 (P=0.01), which was greater for the IBMX- compared to IBMX+ treatment. In both experiments we did not observed any differences in cell morphology among treatments. Our results suggest that neither insulin nor IBMX are required to accelerate redifferentiation process of pig-derived DFAT cells.
Utilization of castor bean meal treated with calcium hydroxide, fed wet or dry, by lambs
(Livestock Science, 2014-10) Gionbelli, T. R. S.; Veloso, C. M.; Gionbelli, M. P.; Novais, M. A. S.; Silva, A. L.; Espechit, C. J. B.; Campos, J. M. S.; Valadares Filho, S. C.; Pereira, O. G.; Cunha, C. S.; Alcântara, P. H.; Virgínio Junior, G. F.; Duarte, M. S.
Effects of replacement of soybean meal by wet or dry castor bean meal (CM) in diets of feedlot lambs on animal performance, carcass traits, intake, digestibility and hepatic function were assessed. Thirty-five mixed-breed woolless lambs averaging 20±0.26 kg body weight were randomly assigned into five treatments in a 2×2+1 factorial design with 7 animals per treatment. Control animals were fed a diet containing soybean meal while lambs from the remaining groups received diets with 50 or 100% level by dry (DCM) or wet (WCM) calcium hydroxide treated CM as a replacement of soybean meal. Lambs were fed for 70 days and slaughtered at the end of the trial for further carcass traits evaluation. Dry matter intake and digestibility, final body weight, average daily gain and carcass weights were not affected (P>0.05) by the substitution of soybean meal by either CM source. Serum levels of aspartate aminotransferase and alanine aminotransferase enzymes did not differ among treatments (P>0.05) indicating no effect of treated CM on hepatic function. These results suggest that castor bean meal treated with calcium hydroxide solution (60 g/kg) can totally replace the soybean meal (up to 18% of dry matter of diet) in the diet of finishing lambs without negative effects on weight gain, intake, digestibility and hepatic function. Castor bean meal treated with calcium hydroxide solution can be fed to animals after 18 h in room temperature, in wet form, without sun or oven-drying.