Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/24672
Tipo: Artigo
Título: Genomic and biological characterization of a new member of the genus Phikmvvirus infecting phytopathogenic Ralstonia bacteria
Autor(es): Xavier, André da Silva
Silva, Fernanda Pereira da
Vidigal, Pedro Marcus Pereira
Lima, Thamylles Thuany Mayrink
Souza, Flavia Oliveira de
Alfenas-Zerbini, Poliane
Abstract: Bacterial wilt caused by Ralstonia spp., soil-borne Gram-negative bacteria, is considered one of the most important plant diseases in tropical and subtropical regions of the world. A large number of bacteriophages capable of lysing or physiologically reprogramming cells of Ralstonia spp. have been reported in Asia. Despite the potential use of these organisms in the management of bacterial wilt, information on viruses that infect Ralstonia spp. is nonexistent in the Americas. We isolated a virus that infects Ralstonia spp. from a soil sample in the state of Minas Gerais, Brazil. Microscopy and genomic and phylogenetic analysis allowed us to classify the virus as a member of the family Podoviridae, genus Phikmvvirus. In spite of its relationship to Ralstonia virus RSB3, an Asian isolate, genomic and biological characteristics showed that the virus isolated in Brazil, tentatively named “Ralstonia virus phiAP1” (phiAP1), belongs to a new species. phiAP1 has EPS depolymerase activity and contains two putative virion-associated peptidoglycan hydrolases (VAPGHs), which reveals a robust mechanism of pathogenesis. Furthermore, phiAP1 specifically infects Ralstonia solanacearum, R. pseudosolanacearum and R. syzygii, causing cell lysis, but it was not able to infect thirteen other bacteria that were tested. Together, these characteristics highlight the biotechnological potential of this virus for the management of bacterial wilt.
Palavras-chave: Genomic and biological characterization
Phikmvvirus infecting
Ralstonia bacteria
Editor: Archives of Virology
Tipo de Acesso: Springer-Verlag GmbH Austria, part of Springer Nature
URI: https://doi.org/10.1007/s00705-018-4006-4
http://www.locus.ufv.br/handle/123456789/24672
Data do documento: Dez-2018
Aparece nas coleções:Fitopatologia - Artigos

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