Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/23318
Tipo: Artigo
Título: Purification and characterization of two new cell-bound bioactive compounds produced by wild Lactococcus lactis strain
Autor(es): Saraiva, Margarete Alice Fontes
Brede, Dag Anders
Nes, Ingolf Figved
Baracat- Pereira, Maria Cristina
Queiroz, Marisa Vieira de
Moraes, Celia Alencar de
Abstract: Novel compounds and innovative methods are required considering that antibiotic resistance has reached a crisis point. In the study, two cell-bound antimicrobial compounds produced by Lactococcus lactis ID1.5 were isolated and partially characterized. Following purification by cationic exchange and a solid-phase C18 column, antimicrobial activity was recovered after three runs of RPC using 60% (v/v) and 100% (v/v) of 2-propanol for elution, suggesting that more than one antimicrobial compound were produced by L. lactis ID1.5, which were in this study called compounds AI and AII. The mass spectrum of AI and AII showed major intensity ions at m/z 1070.05 and 955.9 Da, respectively. The compound AI showed a spectrum of antimicrobial activity mainly against L. lactis species, while the organisms most sensitive to compound AII were Bacillus subtilis, Listeria innocua, Streptococcus pneumoniae and Pseudomonas aeruginosa. The antimicrobial activity of both compounds was suppressed by treatment with Tween 80. Nevertheless, both compounds showed high stability to heat and proteases treatments. The isolated compounds, AI and AII, showed distinct properties from other antimicrobial substances already reported as produced by L. lactis, and have a significant inhibitory effect against two clinically important respiratory pathogens.
Palavras-chave: Antimicrobial compounds
Cell extract
Lactic acid bacteria
Purification
Editor: FEMS Microbiology Letters
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1093/femsle/fnx130
http://www.locus.ufv.br/handle/123456789/23318
Data do documento: Jun-2017
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