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https://locus.ufv.br//handle/123456789/22911
Tipo: | Artigo |
Título: | Protective effect of ions against cell death induced by acid stress in Saccharomyces |
Autor(es): | Sant’Ana, Gilzeane dos Santos Paes, Lisvane da Silva Paiva, Argentino F. Vieira Fietto, Luciano Gomes Totola, Antônio Helvécio Trópia, Maria J. Magalhães Silveira- Lemos, Denise Lucas, Cândida Fietto, Juliana L. Rangel Brandão, Rogelio L. Castro, Ieso de Miranda |
Abstract: | Saccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under these conditions, a pH 2.0 was the main factor responsible for decreased cell viability. Importantly, the addition of low concentrations of sodium chloride (NaCl) protected cells in acidic conditions more effectively than other salts. In the absence of S. boulardii mutants, the protective effects of Na 1 in yeast viability in acidic conditions was tested using S. cerevisiae Na 1 -ATPases (ena1-4), Na 1 /H 1 antiporter (nha1D) and Na 1 /H 1 antiporter prevacuolar (nhx1D) null mutants, respectively. Moreover, we provide evidence suggesting that this protection is determined by the plasma membrane potential, once altered by low pH and low NaCl concentrations. Additionally, the absence or low expression/activity of Ena proteins seems to be closely related to the basal membrane potential of the cells. |
Palavras-chave: | Saccharomyces boulardii Probiotic Acid tolerance ENA genes |
Editor: | FEMS Yeast Research |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1111/j.1567-1364.2009.00523.x http://www.locus.ufv.br/handle/123456789/22911 |
Data do documento: | Ago-2009 |
Aparece nas coleções: | Artigos |
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artigo.pdf | texto completo | 220,07 kB | Adobe PDF | Visualizar/Abrir |
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