Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22911
Tipo: Artigo
Título: Protective effect of ions against cell death induced by acid stress in Saccharomyces
Autor(es): Sant’Ana, Gilzeane dos Santos
Paes, Lisvane da Silva
Paiva, Argentino F. Vieira
Fietto, Luciano Gomes
Totola, Antônio Helvécio
Trópia, Maria J. Magalhães
Silveira- Lemos, Denise
Lucas, Cândida
Fietto, Juliana L. Rangel
Brandão, Rogelio L.
Castro, Ieso de Miranda
Abstract: Saccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under these conditions, a pH 2.0 was the main factor responsible for decreased cell viability. Importantly, the addition of low concentrations of sodium chloride (NaCl) protected cells in acidic conditions more effectively than other salts. In the absence of S. boulardii mutants, the protective effects of Na 1 in yeast viability in acidic conditions was tested using S. cerevisiae Na 1 -ATPases (ena1-4), Na 1 /H 1 antiporter (nha1D) and Na 1 /H 1 antiporter prevacuolar (nhx1D) null mutants, respectively. Moreover, we provide evidence suggesting that this protection is determined by the plasma membrane potential, once altered by low pH and low NaCl concentrations. Additionally, the absence or low expression/activity of Ena proteins seems to be closely related to the basal membrane potential of the cells.
Palavras-chave: Saccharomyces boulardii
Probiotic
Acid tolerance
ENA genes
Editor: FEMS Yeast Research
Tipo de Acesso: Open Access
URI: http://dx.doi.org/10.1111/j.1567-1364.2009.00523.x
http://www.locus.ufv.br/handle/123456789/22911
Data do documento: Ago-2009
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdftexto completo220,07 kBAdobe PDFVisualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.