Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/22580
Tipo: Artigo
Título: In vitro regeneration of triploid plants from mature endosperm culture of commercial passionfruit (Passiflora edulis Sims)
Autor(es): Otoni, Wagner Campos
Antoniazzi, Camila Aparecida
Faria, Rodrigo Brito de
Carvalho, Paula Pinheiro de
Mikovski, Andreia Izabel
Carvalho, Ilio Fealho de
Matos, Elyabe Monteiro de
Reis, Aryane Campos
Viccin, Lyderson Facio
Pinto, Daniela Lopes Paim
Rocha, Diego Ismael
Silva, Maurecilne Lemes da
Abstract: Due to the triploid nature of endosperm, an embryonic reserve tissue, in vitro culture of endosperm tissues has been considered a direct method for production of polyploids. In the present study, we report the establishment of an in vitro regeneration system from endosperm culture for production of triploid Passiflora edulis plants, the main commercial species of passionfruit. Surface-sterilized endosperms were cultured in MS medium with different concentrations of benzyladenine (BA), thidiazuron (TDZ), and kinetin (KIN). The cultures were maintained in a growth chamber under controlled conditions, for 60 days. Thidiazuron was the only type of cytokinin that induced shoot production in the endosperm tissues; the highest number of shoots was produced in the presence of 4.5 and 9.0 μM TDZ. Flow cytometry and chromosomal analysis confirmed that endosperm-derived plants were triploid. The internal standard, Pisum sativum, and the diploid control, seed-derived Passiflora edulis plants (2n = 2× = 18), showed average DNA quantities of 9.09 and 3.35 pg respectively. Endosperm-derived P. edulis plants showed an average DNA content of 5.10 pg and a chromosome count of 27 (3n = 3× = 27), the same ploidy level as the endosperm (triploid). Our data open new prospects for breeding of passionfruit by means of a stable and reproducible regeneration system from endosperm culture leading to generation of triploid plants.
Palavras-chave: Endosperm culture
Flow cytometry
In vitro organogenesis
Ploidy manipulation
Polyploidy
Editor: Scientia Horticulturae
Tipo de Acesso: 2018 Elsevier B.V. All rights reserved.
URI: https://doi.org/10.1016/j.scienta.2018.05.001
http://www.locus.ufv.br/handle/123456789/22580
Data do documento: 19-Ago-2018
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