Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/22580
Tipo: | Artigo |
Título: | In vitro regeneration of triploid plants from mature endosperm culture of commercial passionfruit (Passiflora edulis Sims) |
Autor(es): | Otoni, Wagner Campos Antoniazzi, Camila Aparecida Faria, Rodrigo Brito de Carvalho, Paula Pinheiro de Mikovski, Andreia Izabel Carvalho, Ilio Fealho de Matos, Elyabe Monteiro de Reis, Aryane Campos Viccin, Lyderson Facio Pinto, Daniela Lopes Paim Rocha, Diego Ismael Silva, Maurecilne Lemes da |
Abstract: | Due to the triploid nature of endosperm, an embryonic reserve tissue, in vitro culture of endosperm tissues has been considered a direct method for production of polyploids. In the present study, we report the establishment of an in vitro regeneration system from endosperm culture for production of triploid Passiflora edulis plants, the main commercial species of passionfruit. Surface-sterilized endosperms were cultured in MS medium with different concentrations of benzyladenine (BA), thidiazuron (TDZ), and kinetin (KIN). The cultures were maintained in a growth chamber under controlled conditions, for 60 days. Thidiazuron was the only type of cytokinin that induced shoot production in the endosperm tissues; the highest number of shoots was produced in the presence of 4.5 and 9.0 μM TDZ. Flow cytometry and chromosomal analysis confirmed that endosperm-derived plants were triploid. The internal standard, Pisum sativum, and the diploid control, seed-derived Passiflora edulis plants (2n = 2× = 18), showed average DNA quantities of 9.09 and 3.35 pg respectively. Endosperm-derived P. edulis plants showed an average DNA content of 5.10 pg and a chromosome count of 27 (3n = 3× = 27), the same ploidy level as the endosperm (triploid). Our data open new prospects for breeding of passionfruit by means of a stable and reproducible regeneration system from endosperm culture leading to generation of triploid plants. |
Palavras-chave: | Endosperm culture Flow cytometry In vitro organogenesis Ploidy manipulation Polyploidy |
Editor: | Scientia Horticulturae |
Tipo de Acesso: | 2018 Elsevier B.V. All rights reserved. |
URI: | https://doi.org/10.1016/j.scienta.2018.05.001 http://www.locus.ufv.br/handle/123456789/22580 |
Data do documento: | 19-Ago-2018 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | texto completo | 2,38 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.