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https://locus.ufv.br//handle/123456789/22040
Tipo: | Artigo |
Título: | Protocols for the isolation and detection of lactic acid bacteria with bacteriocinogenic potential |
Autor(es): | Moraes, Paula Mendonça Perin, Luana Martins Perin Ortolani, Maria Beatriz Tassinari Yamazi, Anderson Keizo Viçosa, Gabriela Nogueira Nero, Luís Augusto |
Abstract: | The objective of this study was to evaluate culture media and methodologies for isolation and detection of lactic acid bacteria (LAB) capable to produce bacteriocin-like substances. Samples of milk and cheese were pour plated on de Mann-Rogosa-Sharpe agar (MRS) and Kang-Fung-Sol agar (KFS) (both at 35 °C/48 h, under anaerobiosis), from which 389 and 256 LAB cultures were selected. The antagonistic activity of them was evaluated using the spot-on-the-lawn and two culture media: brain-heart infusion agar with catalase (BHI + C) and M17 (both at 35 °C/24 h). The proteinaceous nature of the antagonistic cultures was verified using: spot-on-the-lawn (MRS, 25 °C/24 h, under anaerobiosis) and well-diffusion (cultures amplified on modified MRS broth at 25 °C/24 h, and then neutralized using NaOH). Listeria monocytogenes ATCC 7644 was used as indicator. A larger number of antagonist cultures were isolated from MRS (83 by M17 and 65 by BHI + C) in comparison to KFS (24 by M17 and 15 by BHI + C). The spot-on-the-lawn identified a higher frequency of LAB capable of producing bacteriocin-like substances. MRS was considered to be the best culture media for the isolation of LAB capable to produce bacteriocin-like substances, activity that was better identified using the spot-on-the-lawn methodology. |
Palavras-chave: | Lactic acid bacteria Bacteriocins Analytical methods Spot-on-the-lawn Well-diffusion assay |
Editor: | LWT - Food Science and Technology |
Tipo de Acesso: | Elsevier Ltd. |
URI: | https://doi.org/10.1016/j.lwt.2010.05.005 http://www.locus.ufv.br/handle/123456789/22040 |
Data do documento: | Nov-2010 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | texto completo | 200,31 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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