Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/21899
Tipo: Artigo
Título: Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity
Autor(es): Sousa, Tiago Vieira
Caixeta, Eveline Teixeira
Alkimim, Emilly Ruas
Oliveira, Antonio Carlos Baião de
Pereira, Antonio Alves
Zambolim, Laércio
Sakiyama, Ney Sussumu
Abstract: New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes using morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.
Palavras-chave: Fingerprinting
Molecular profiling
Genetic diversity
Cultivar identification
Protection of plant cultivars
Cultivars register
SSR marker
Editor: Euphytica
Tipo de Acesso: Springer Nature Switzerland AG.
URI: http://dx.doi.org/10.1007/s10681-017-1865-9
http://www.locus.ufv.br/handle/123456789/21899
Data do documento: 3-Mar-2017
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