Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/21484
Tipo: Artigo
Título: Growth conditions of clostridium perfringens type B for production of toxins used to obtain veterinary vaccines
Autor(es): Passos, Flavia Maria Lopes
Brandi, Igor Viana
Mozzer, Otto Domenici
Jorge, Edson Vander
Passos, Frederico Jose Vieira
Cangussu, Alex Sander Rodrigues
Sobrinho, Eliane Macedo
Abstract: The diseases caused for Clostridium perfringens are generically called enterotoxemias because toxins produced in the intestine may be absorbed into the general circulation. C. perfringens type B, grown in batch fermentation, produced toxins used to obtain veterinary vaccines. Glucose in concentrations of 1.4–111.1 mM was used to define the culture medium. The minimum concentration for a satisfactory production of vaccines against clostridial diseases was 55.6 mM. Best results were brought forth by meat and casein peptones, both in the concentration 5.0 g l^−1 in combination with glucose and a culture pH maintained at 6.5 throughout the fermentation process. The production of lactic, acetic and propionic organic acids was observed. Ethanol was the metabolite produced in the highest concentration when cultures maintained steady pH of 6.5 with exception of cultures with initial glucose concentration of 1.4 mM, where the highest production was of propionic acid. Maximal cell concentration and the highest toxin title concomitantly low yield coefficient to organic acids and ethanol were obtained using basal medium containing 111.1 mM glucose under a controlled pH culture (pH) 6.5 in batch fermentations of C. perfringens type B. These data contribute to improve process for industrial toxin production allowing better condition to produce a toxoid vaccine.
Palavras-chave: Clostridium perfringens
Growth conditions
Anaerobiosis
Toxin
Veterinary vaccine
Editor: Bioprocess and Biosystems Engineering
Tipo de Acesso: Springer-Verlag Berlin Heidelberg 2014
URI: http://dx.doi.org/10.1007/s00449-014-1146-0
http://www.locus.ufv.br/handle/123456789/21484
Data do documento: 27-Fev-2014
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