Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19880
Tipo: Artigo
Título: Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression
Autor(es): Vieira, Lorena Melo
Rocha, Diego Ismael
Pinto, Daniela Lopes Paim
Tanaka, Francisco André Ossamu
Dornelas, Marcelo Carnier
Otoni, Wagner Campos
Abstract: The integration of cellular and molecular data is essential for understanding the mechanisms involved in the acquisition of competence by plant somatic cells and the cytological changes that underlie this process. In the present study, we investigated the dynamics and fate of Passiflora edulis Sims cotyledon explants that were committed to somatic embryogenesis by characterizing the associated ultrastructural events and analysing the expression of a putative P. edulis ortholog of the Somatic Embryogenesis Receptor-like Kinase (SERK) gene. Embryogenic calli were obtained from zygotic embryo explants cultured on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Callus formation was initiated by the division of cells derived from the protodermal and subprotodermal cells on the abaxial side of the cotyledons. The isodiametric protodermal cells of the cotyledon explants adopted a columnar shape and became meristematic at the onset of PeSERK expression, which was not initially detected in explant cells. Therefore, we propose that these changes represent the first observable steps towards the acquisition of a competent state within this regeneration system. PeSERK expression was limited to the early stages of somatic embryogenesis; the expression of this gene was confined to proembryogenic zones and was absent in the embryos after the globular stage. Our data also demonstrated that the dynamics of the mobilization of reserve compounds correlated with the differentiation of the embryogenic callus.
Palavras-chave: Cellular competence
In situ hybridization
Reserve mobilization
Reserve mobilization
Somatic embryogenesis
SERK gene
Ultrastructure
Editor: Protoplasma
Tipo de Acesso: Springer-Verlag Wien
URI: http://dx.doi.org/10.1007/s00709-015-0837-y
http://www.locus.ufv.br/handle/123456789/19880
Data do documento: 26-Mai-2015
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