Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/19852
Tipo: | Artigo |
Título: | Debaryomyces hansenii UFV-1 intracellular α-Galactosidase characterization and comparative studies with the extracellular enzyme |
Autor(es): | Rezende, Sebastião T. de Viana, Pollyanna A. Passos, Flávia Maria Lopes Oliveira, Jamil S. Teixeira, Kádima N. Santos, Alexandre M. C. Bemquerer, Marcelo P. Rosa, José C. Santoro, Marcelo M. Guimarães, Valéria M. |
Abstract: | Debaryomyces hansenii cells cultivated on galactose produced extracellular and intracellular α-galactosidases, which showed 54.5 and 54.8 kDa molecular mass (MALDI-TOF), 60 and 61 kDa (SDS−PAGE) and 5.15 and 4.15 pI values, respectively. The extracellular and intracellular deglycosylated forms presented 36 and 40 kDa molecular mass, with 40 and 34% carbohydrate content, respectively. The N-terminal sequences of the α-galactosidases were identical. Intracellular α-galactosidase showed smaller thermostability when compared to the extracellular enzyme. D. hansenii UFV-1 extracellular α-galactosidase presented higher kcat than the intracellular enzyme (7.16 vs 3.29 s^−1, respectively) for the p-nitrophenyl-α-d-galactopyranoside substrate. The Km for hydrolysis of pNPαGal, melibiose, stachyose, and raffinose were 0.32, 2.12, 10.8, and 32.8 mM, respectively. The intracellular enzyme was acompetitively inhibited by galactose (Ki = 0.70 mM), and it was inactivated by Cu(II) and Ag(I). Enzyme incubation with soy milk for 6 h at 55 °C reduced stachyose and raffinose amounts by 100 and 73%, respectively. |
Palavras-chave: | Characterization Debaryomyces hansenii UFV-1 Deglycosylation Galacto-oligosaccharides α-Galactosidases |
Editor: | Journal of Agricultural and Food Chemistry |
Tipo de Acesso: | American Chemical Society |
URI: | http://dx.doi.org/10.1021/jf8030919 15205118 http://www.locus.ufv.br/handle/123456789/19852 |
Data do documento: | 18-Fev-2009 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | texto completo | 992,39 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.