Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19649
Tipo: Artigo
Título: Melipona quadrifasciata (Hymenoptera: Apidae) fat body persists through metamorphosis with a few apoptotic cells and an increased autophagy
Autor(es): Santos, Douglas Elias
Azevedo, Dihego Oliveira
Campos, Lúcio Antônio Oliveira
Zanuncio, José Cola
Serrão, José Eduardo
Abstract: Fat body, typically comprising trophocytes, provides energy during metamorphosis. The fat body can be renewed once the larval phase is complete or recycled and relocated to form the fat body of the adult insect. This study aims to identify the class of programmed cell death that occurs within the fat body cells during the metamorphosis of the stingless bee Melipona quadrifasciata. Using immunodetection techniques, the fat body of the post-defecating larvae and the white-, pink-, brown-, and black-eyed pupae were tested for cleaved caspase-3 and DNA integrity, followed by ultrastructural analysis and identification of autophagy using RT-PCR for the Atg1 gene. The fat body of M. quadrifasciata showed some apoptotic cells positive for cleaved caspase-3, although without DNA fragmentation. During development, the fat body cells revealed an increased number of mitochondria and free ribosomes, in addition to higher amounts of autophagy Atg1 mRNA, than that of the pupae. The fat body of M. quadrifasciata showed few cells which underwent apoptosis, but there was evidence of increased autophagy at the completion of the larval stage. All together, these data show that some fat body cells persist during metamorphosis in the stingless bee M. quadrifasciata.
Palavras-chave: Autophagy
Cell death
Development
Insect
Editor: Protoplasma
Tipo de Acesso: Springer-Verlag Wien
URI: http://dx.doi.org/10.1007/s00709-014-0707-z
http://www.locus.ufv.br/handle/123456789/19649
Data do documento: 1-Out-2014
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