Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/19604
Tipo: | Artigo |
Título: | Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus |
Autor(es): | Pereira, Jorge Fernando Almeida, Ana Paula Morais Martins Cota, Júnio Pamphile, João Alencar Silva, Gilvan Ferreira da Araújo, Elza Fernandes de Gramacho, Karina Peres Brommonschenkel, Sérgio Hermı́nio Pereira, Gonçalo Amarante Guimarães Queiroz, Marisa Vieira de |
Abstract: | Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6–12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus. |
Palavras-chave: | Moniliophthora perniciosa PIF/Harbinger |
Editor: | Microbiology |
Tipo de Acesso: | SGM |
URI: | https://doi.org/10.1099/mic.0.062901-0 http://www.locus.ufv.br/handle/123456789/19604 |
Data do documento: | 24-Out-2012 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | Texto completo | 900,25 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.