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https://locus.ufv.br//handle/123456789/19373
Tipo: | Artigo |
Título: | Mobilisation of bacteria in a fine-grained residual soil by electrophoresis |
Autor(es): | Rocha, Ulisses Nunes Da Tótola, Marcos Rogério Pessoa, Denise Maria Mano Araruna Júnior, José Tavares Neves, Júlio César Lima Borges, Arnaldo Chaer |
Abstract: | An investigation of electrokinetic bacterial mobilisation in a residual soil from gneiss is presented here. The experimental program aimed at assessing the efficacy of electrophoresis against the electro-osmotic flow to transport endospores of Bacillus subtilis LBBMA 155 and nitrogen-starved cells of Pseudomonas sp. LBBMA 81. Electrokinesis was performed on a low hydraulic reconstituted clayey soil column submitted to a 5 mA electrical current for 24 h. Cells were coccoid-shaped and characterised as possessing low surface hydrophobicity and less than 1 μm in diameter. Distribution coefficient for B. subtilis in the soil was between 16.8 and 19.9 times higher than that for Pseudomonas sp. Distribution coefficient for B. subtilis between eluate and anionic exchange column was 11.8 times higher than that for Pseudomonas sp. After the electrokinesis, it was shown that cells and endospores were distributed hyperbolically through the soil probe and moved against the electro-osmotic flow; however, endospores were transported throughout all soil core and starved cells only till half of its length. The higher transport efficiency of B. subtilis endospores was attributed to their higher negative charge on cell surface. These results demonstrate that electrokinesis can be used for bacteria transport in soils with low hydraulic conductivity, even against the electro-osmotic flow. |
Palavras-chave: | Electrokinesis Bacterial electrophoresis Cell transport Bacterial starvation |
Editor: | Journal of Hazardous Materials |
Tipo de Acesso: | Elsevier B.V. |
URI: | https://doi.org/10.1016/j.jhazmat.2008.04.022 http://www.locus.ufv.br/handle/123456789/19373 |
Data do documento: | 15-Jan-2009 |
Aparece nas coleções: | Artigos |
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artigo.pdf Until 2100-12-31 | texto completo | 645,13 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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