Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19373
Tipo: Artigo
Título: Mobilisation of bacteria in a fine-grained residual soil by electrophoresis
Autor(es): Rocha, Ulisses Nunes Da
Tótola, Marcos Rogério
Pessoa, Denise Maria Mano
Araruna Júnior, José Tavares
Neves, Júlio César Lima
Borges, Arnaldo Chaer
Abstract: An investigation of electrokinetic bacterial mobilisation in a residual soil from gneiss is presented here. The experimental program aimed at assessing the efficacy of electrophoresis against the electro-osmotic flow to transport endospores of Bacillus subtilis LBBMA 155 and nitrogen-starved cells of Pseudomonas sp. LBBMA 81. Electrokinesis was performed on a low hydraulic reconstituted clayey soil column submitted to a 5 mA electrical current for 24 h. Cells were coccoid-shaped and characterised as possessing low surface hydrophobicity and less than 1 μm in diameter. Distribution coefficient for B. subtilis in the soil was between 16.8 and 19.9 times higher than that for Pseudomonas sp. Distribution coefficient for B. subtilis between eluate and anionic exchange column was 11.8 times higher than that for Pseudomonas sp. After the electrokinesis, it was shown that cells and endospores were distributed hyperbolically through the soil probe and moved against the electro-osmotic flow; however, endospores were transported throughout all soil core and starved cells only till half of its length. The higher transport efficiency of B. subtilis endospores was attributed to their higher negative charge on cell surface. These results demonstrate that electrokinesis can be used for bacteria transport in soils with low hydraulic conductivity, even against the electro-osmotic flow.
Palavras-chave: Electrokinesis
Bacterial electrophoresis
Cell transport
Bacterial starvation
Editor: Journal of Hazardous Materials
Tipo de Acesso: Elsevier B.V.
URI: https://doi.org/10.1016/j.jhazmat.2008.04.022
http://www.locus.ufv.br/handle/123456789/19373
Data do documento: 15-Jan-2009
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