Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19339
Tipo: Artigo
Título: Controlling cooperativity in β-cyclodextrin–DNA binding reactions
Autor(es): Rocha, M. S.
Alves, P. S.
Mesquita, O. N.
Abstract: We have investigated the interaction between the native neutral β- cyclodextrin (CD) and the DNA molecule by performing single-molecule stretching experiments with optical tweezers. In particular, we have monitored the changes of the mechanical properties of the CD−DNA complexes as a function of the CD concentration in the sample. By using a quenched disorder statistical model, we were also capable to extract important physicochemical information (equilibrium binding constants, cooperativity degree) of such interaction from the mechanical data. In addition, we have found that the interaction occurs by two different mechanisms, first with the formation of relatively large CD clusters along the double helix, which thereafter can locally denature the DNA molecule by forming hydrogen bonds with the base pairs that eventually flip out. A prediction of our quenched disorder model was that cooperativity could be controlled by adjusting the surface charge of β-CD molecules. This prediction is confirmed in the present work.
Palavras-chave: Cooperative binding
Cyclodextrin
DNA
Persistence length
Editor: The Journal of Physical Chemistry Letters
Tipo de Acesso: American Chemical Society
URI: http://dx.doi.org/10.1021/acs.jpclett.5b01603
http://www.locus.ufv.br/handle/123456789/19339
Data do documento: 26-Ago-2015
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