Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/18718
Tipo: Artigo
Título: A cooperative transition from the semi-flexible to the flexible regime of polymer elasticity: Mitoxantrone-induced DNA condensation
Autor(es): Lima, C.H.M.
Almeida, G.O.
Rocha, M.S.
Abstract: We report a high cooperative transition from the semi-flexible to the flexible regime of polymer elasticity during the interaction of the DNA molecule with the chemotherapeutic drug Mitoxantrone (MTX). By using single molecule force spectroscopy, we show that the force-extension curves of the DNA-MTX complexes deviate from the typical worm-like chain behavior as the MTX concentration in the sample increases, becoming straight lines for sufficiently high drug concentrations. The behavior of the radius of gyration of the complexes as a function of the bound MTX concentration was used to quantitatively investigate the cooperativity of the condensation process. The present methodology can be promptly applied to other ligands that condense the DNA molecule upon binding, opening new possibilities in the investigation of this type of process and, more generally, in the investigation of phase transitions in polymer physics.
Palavras-chave: DNA condensation
Mitoxantrone
Polymer elasticity
Force spectroscopy
Editor: Biochimica et Biophysica Acta (BBA) - General Subjects
Tipo de Acesso: Open Access
URI: https://doi.org/10.1016/j.bbagen.2018.01.019
http://www.locus.ufv.br/handle/123456789/18718
Data do documento: 30-Jan-2018
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