Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/18274
Tipo: | Artigo |
Título: | Effect of the inhibitors phenylalanine arginyl ß-naphthylamide (PAßN) and 1-(1-naphthylmethyl)-piperazine (NMP) on expression of genes in multidrug efflux systems of Escherichia coli isolates from bovine mastitis |
Autor(es): | Barrero, M.A. Ospina Pietralonga, P.A.G. Schwarz, D.G.G. Silva Junior, A. Paula, S.O. Moreira, M.A.S. |
Abstract: | The multidrug efflux system in bacteria can reduce antibiotic concentration inside the cell, leading to failure in the treatment of bacterial diseases. This study evaluated the influence of two efflux pump inhibitors (EPIs), phenylalanine arginyl ß-naphthylamide (PAßN) and 1-(1-naphthylmethyl)-piperazine (NMP), on the gene expression of three multidrug efflux systems, AcrAB, AcrEF and EmrAB in Escherichia coli bovine mastitis isolates resistant to ampicillin and sulfamethoxazole/trimethoprim simultaneously. Each isolate had at least three multidrug efflux system genes. The acrA and acrB had the lowest expression levels in all treatments, while the emrA or emrB showed the highest expression levels in the presence of ampicillin, sulfamethoxazole/trimethoprim, PAβN and NMP. EPIs also contributed to the decrease in arcF expression when used in combination with ampicillin treatment. Since PAßN showed stronger effects than NMP, it may serve as an alternative to assist in the antimicrobial therapy of mastitis. |
Palavras-chave: | Milk Multiresistant bacteria Therapy Antimicrobial |
Editor: | Research in Veterinary Science |
Tipo de Acesso: | Elsevier Ltd |
URI: | https://doi.org/10.1016/j.rvsc.2014.05.013 http://www.locus.ufv.br/handle/123456789/18274 |
Data do documento: | 25-Mai-2014 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | Texto completo | 565,95 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.