Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/13991
Tipo: Artigo
Título: Bacteriocinogenic and virulence potential of Enterococcus isolates obtained from raw milk and cheese
Autor(es): Moraes, P.M.
Perin, L.M.
Todorov, S.D.
Silva Jr, A.
Franco, B.D.G.M.
Nero, L.A.
Abstract: To provide molecular and phenotypical characterization of Enterococcus isolates obtained from raw milk and cheese, regarding their bacteriocinogenic and virulence activity. Forty-three bacteriocinogenic enterococci isolates were identified by 16s rDNA, fingerprinted by RAPD-PCR analysis and tested by PCR for the presence of genes for lantibiotics (lanM, lanB and lanC) and enterocins (entA, entB, entP, entL50AB and entAS48) and by phenotypical methods for bacteriocin production and inhibitory spectrum. Also, the virulence of the isolates was evaluated by PCR for genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for gelatinase, lipase, DNAse and α- and β-haemolysis. Most isolates (93·0%) harboured at least one lantibiotic or enterocin gene and were positive for several tested virulence genes, mainly asa1 (100%), gelE (93·0%) and efaA (83·7%). 53·5% of the isolates presented β-haemolysis. Enterococcus spp. isolates presented an interesting potential application for food preservation because of bacteriocin production; however, virulence-related genes were identified in all RAPD profiles. The study demonstrated the contradictory characteristics of the tested Enterococcus isolates: they presented a good potential for application in food biopreservation but contained several virulence factors.
Palavras-chave: Bacteriocins
Cheese
Enterococcus
Milk
PCR
RAPD
Virulence
Editor: Journal of Applied Microbiology
Tipo de Acesso: Open Access
URI: https://doi.org/10.1111/j.1365-2672.2012.05341.x
http://www.locus.ufv.br/handle/123456789/13991
Data do documento: 9-Mai-2012
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