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https://locus.ufv.br//handle/123456789/13606
Tipo: | Artigo |
Título: | Purification and characterization of trypsin produced by gut bacteria from Anticarsia gemmatalis |
Autor(es): | Pilon, Franciny Martins Silva, Camila da Rocha Visôtto, Liliane Evangelista Barros, Rafael de Almeida Silva Júnior, Neilier Rodrigues da Campos, Wellington Garcia Oliveira, Maria Goreti de Almeida |
Abstract: | Purification of active trypsin in the digestive process of insects is essential for the development of potent protease inhibitors (PIs) as an emerging pest control technology and research into insect adaptations to dietary PIs. An important aspect is the presence of proteolytic microorganisms, which contribute to host nutrition. Here, we purified trypsins produced by bacteria Bacillus cereus, Enterococcus mundtii, Enterococcus gallinarum, and Staphylococcus xylosus isolated from the midgut of Anticarsia gemmatalis. The trypsins had a molecular mass of approximately 25 kDa. The enzymes showed increased activity at 40°C, and they were active at pH values 7.5–10. Aprotinin, bis-benzamidine, and soybean Kunitz inhibitor (SKTI) significantly inhibited trypsin activity. The l-1-tosyl-amido-2-phenylethylchloromethyl ketone (TPCK), pepstatin A, E-64, ethylenediamine tetraacetic acid, and calcium ions did not affect the enzyme activity at the concentrations tested. We infer the purified trypsins do not require calcium ions, by which they differ from the trypsins of other microorganisms and the soluble and insoluble trypsins characterized from A. gemmatalis. These data suggest the existence of different isoforms of trypsin in the velvetbean caterpillar midguts. |
Palavras-chave: | Bacteria Pest control Protease inhibitor Trypsin |
Editor: | Archives of Insect Biochemistry and Physiology |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1002/arch.21407 http://www.locus.ufv.br/handle/123456789/13606 |
Data do documento: | 1-Ago-2017 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Pilon_et_al-2017-Archives_of_Insect_Biochemistry_and_Physiology.pdf | Texto completo | 1,05 MB | Adobe PDF | Visualizar/Abrir |
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