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https://locus.ufv.br//handle/123456789/13603
Tipo: | Artigo |
Título: | Association of vitamin e with rapid thawing on goat semen |
Autor(es): | Penitente-Filho, Jurandy Mauro Oliveira, Fabrício Albani Jimenez, Carolina Rodriguez Carrascal, Erly Dias, Júlio César Oliveira Oliveira, Gisele Dias Silveira, Renata Gomes Silveira, Camila Oliveira Torres, Ciro Alexandre Alves |
Abstract: | The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5 ∘ C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155 ∘ C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196 ∘ C). Straws were thawed at 38 ∘ C/60 seconds or at 60 ∘ C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm⋅Kg −1 ) followed by incubation during 60 minutes in water bath (38 ∘ C). Vitamin E did not affect any studied parameters (P > 0.05). Nevertheless, defrosting rate of 60 ∘ C/7 seconds improved sperm membrane functional integrity (P < 0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process. |
Palavras-chave: | Semen Vitamin E |
Editor: | The Scientific World Journal |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1155/2014/964172 http://www.locus.ufv.br/handle/123456789/13603 |
Data do documento: | 11-Mai-2014 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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964172.pdf | Texto completo | 1,24 MB | Adobe PDF | Visualizar/Abrir |
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