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https://locus.ufv.br//handle/123456789/12018
Tipo: | Artigo |
Título: | Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro |
Autor(es): | Bastos, Matheus Silva e Souza, Luciana Ângelo de Onofre, Thiago Souza Almeida, Márcia Rogéria de Bressan, Gustavo Costa Fietto, Juliana Lopes Rangel Silva Júnior, Abelardo |
Abstract: | Gene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells. |
Palavras-chave: | Leishmania braziliensis Green fluorescent protein Drug screening |
Editor: | Memórias do Instituto Oswaldo Cruz |
Tipo de Acesso: | Open access |
URI: | http://dx.doi.org/10.1590/0074-02760160237 http://www.locus.ufv.br/handle/123456789/12018 |
Data do documento: | 20-Out-2016 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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0074-0276-mioc-112-2-0155.pdf | texto completo | 772,33 kB | Adobe PDF | Visualizar/Abrir |
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