Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/12018
Tipo: Artigo
Título: Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
Autor(es): Bastos, Matheus Silva e
Souza, Luciana Ângelo de
Onofre, Thiago Souza
Almeida, Márcia Rogéria de
Bressan, Gustavo Costa
Fietto, Juliana Lopes Rangel
Silva Júnior, Abelardo
Abstract: Gene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays. Our results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. Transfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
Palavras-chave: Leishmania braziliensis
Green fluorescent protein
Drug screening
Editor: Memórias do Instituto Oswaldo Cruz
Tipo de Acesso: Open access
URI: http://dx.doi.org/10.1590/0074-02760160237
http://www.locus.ufv.br/handle/123456789/12018
Data do documento: 20-Out-2016
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