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https://locus.ufv.br//handle/123456789/24032
Tipo: | Artigo |
Título: | Sugar transport systems in Kluyveromyces marxianus CCT 7735 |
Autor(es): | Silveira, Fernando Augusto da Diniz, Raphael Hermano S. Silveira, Wendel B. da Sampaio, Geraldo M. S. Brandão, Rogelio L. Castro, Ieso M. |
Abstract: | The pattern of glucose repression in most Kluyveromyces marxianus strains does not correlate with fermentative behaviour; however, glucose repression and fermentative metabolism appear to be linked to the kinetics of sugar uptake. In this work, we show that lactose transport in K. marxianus CCT 7735 by lactose-grown cells is mediated by a low-affinity H+-sugar symporter. This system is glucose repressed and able to transport galactose with low affinity. We also observed the activity of a distinct lactose transporter in response to raffinose. Regarding glucose uptake, specificities of at least three low-affinity systems rely on the carbon source available in a given growth medium. Interestingly, it was observed only one high-affinity system is able to transport both glucose and galactose. We also showed that K. marxianus CCT 7735 regulates the expression of sugar transport systems in response to glucose availability. |
Palavras-chave: | Glucose Repression K marxianus Lactose Metabolism regulation Sugar uptake |
Editor: | Antonie van Leeuwenhoek |
Tipo de Acesso: | Springer Nature Switzerland AG |
URI: | https://doi.org/10.1007/s10482-018-1143-4 http://www.locus.ufv.br/handle/123456789/24032 |
Data do documento: | Fev-2019 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 976,67 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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