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https://locus.ufv.br//handle/123456789/19644
Tipo: | Artigo |
Título: | Terminal repeat retrotransposons as DNA markers in fungi |
Autor(es): | Santana, Mateus Ferreira Batista, Aline Duarte Ribeiro, Lílian Emídio Araújo, Elza Fernandes de Queiroz, Marisa Vieira de |
Abstract: | In this study, we demonstrate that ClIRAP primers designed using the transposable element RetroCl1 sequence from Colletotrichum lindemuthianum can be used to generate an efficient IRAP (inter‐retrotransposon amplified polymorphism) molecular marker to study intra‐ and inter‐species diversity in fungi. It has been previously demonstrated that primers generated from this TRIM‐like element can be used in the Colletotrichum species. We now prove that the RetroCl1 sequence can also be used to analyze diversity in different fungi. IRAP profiles were successfully generated for 27 fungi species from 11 different orders, and intra‐species genetic variability was detected in six species. The ClIRAP primers facilitate the use of the IRAP technique for a variety of fungi without prior knowledge of the genome. |
Palavras-chave: | IRAP Molecular marker Retrotransposon TRIM |
Editor: | Journal of Basic Microbiology |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1002/jobm.201200453 http://www.locus.ufv.br/handle/123456789/19644 |
Data do documento: | 26-Fev-2013 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
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artigo.pdf | texto completo | 241,64 kB | Adobe PDF | Visualizar/Abrir |
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