Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/12562
Tipo: | Artigo |
Título: | The dsRNA virus papaya meleira virus and an ssRNA virus are associated with papaya sticky disease |
Autor(es): | Godinho, Marcio Tadeu Amaral, Josiane G. Zerbini, Francisco Murilo Souza, Flávia O. Zerbini, Poliane Alfenas Antunes, Tathiana Ferreira Sá Amaral, Raquel J. Vionette Ventura, José Aires Fernandes, Patricia Machado Bueno |
Abstract: | Papaya sticky disease, or “meleira”, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses ten- tatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 dis- play 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purifi- cation in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants show- ing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymp- tomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2. |
Palavras-chave: | The dsRNA virus Papaya meleira virus Papaya sticky disease |
Editor: | Plos One |
Tipo de Acesso: | Open Access |
URI: | https://doi.org/10.1371/journal.pone.0155240 http://www.locus.ufv.br/handle/123456789/12562 |
Data do documento: | 11-Mai-2016 |
Aparece nas coleções: | Fitopatologia - Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
journal.pone.0155240.PDF | texto completo | 1,57 MB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.