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https://locus.ufv.br//handle/123456789/12290
Tipo: | Artigo |
Título: | Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae) |
Autor(es): | Lopes, Denilce Meneses Campos, Lúcio Antônio de Oliveira Salomão, Tânia Maria Fernandes Tavares, Mara Garcia |
Abstract: | Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers. |
Palavras-chave: | Melipona Stingless bees Microsatellite Transferability |
Editor: | Genetics and Molecular Biology |
Tipo de Acesso: | Open Access |
URI: | http://dx.doi.org/10.1590/S1415-47572010005000017 http://www.locus.ufv.br/handle/123456789/12290 |
Data do documento: | 14-Out-2009 |
Aparece nas coleções: | Artigos |
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gmb-33-2-390.pdf | texto completo | 61,28 kB | Adobe PDF | Visualizar/Abrir |
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