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https://locus.ufv.br//handle/123456789/24107
Tipo: | Artigo |
Título: | In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia |
Autor(es): | Sufiate, Bruna Araújo, Jackson Victor de Queiroz, José Humberto de Gouveia, Angélica S Cardoso, Evandro Moreira, Samara Silveira Soares, Filippe Elias de Freitas Braga, Fabio Ribeiro |
Abstract: | The objective of this study was to purify, characterize, and phylogenetically and structurally analyze the dextranase produced by the fungus Pochonia chlamydosporia. Dextranase produced by the fungus P. chlamydosporia was purified to homogeneity in two steps, with a yield of 152%, purification factor of 6.84 and specific activity of 358.63 U/mg. Its molecular weight was estimated by SDS-PAGE at 64 kDa. The enzyme presented higher activity at 50 °C and pH 5.0, using 100 mM citrate–phosphate buffer, was inhibited by Ag1+, Hg2+, Cu2+, Mg2+, and presented KM of 23.60 µM. Mature dextranase is composed of 585 amino acids residues, with a predicted molecular weight of 64.38 kDa and pI 5.96. This dextranase showed a strong phylogenetic similarity when compared to Trichoderma harzianum dextranase. Its structure consists of two domains: the first composed by 15 β strands, and the second composed by a right-handed parallel β-helix. |
Palavras-chave: | Enzyme Verticillium chlamydosporium Purification 3D structure |
Editor: | 3 Biotech |
Tipo de Acesso: | Springer-Verlag GmbH Germany, part of Springer Nature |
URI: | https://doi.org/10.1007/s13205-018-1192-4 http://www.locus.ufv.br/handle/123456789/24107 |
Data do documento: | Mar-2018 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 1,95 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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