Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/24107
Tipo: Artigo
Título: In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia
Autor(es): Sufiate, Bruna
Araújo, Jackson Victor de
Queiroz, José Humberto de
Gouveia, Angélica S
Cardoso, Evandro
Moreira, Samara Silveira
Soares, Filippe Elias de Freitas
Braga, Fabio Ribeiro
Abstract: The objective of this study was to purify, characterize, and phylogenetically and structurally analyze the dextranase produced by the fungus Pochonia chlamydosporia. Dextranase produced by the fungus P. chlamydosporia was purified to homogeneity in two steps, with a yield of 152%, purification factor of 6.84 and specific activity of 358.63 U/mg. Its molecular weight was estimated by SDS-PAGE at 64 kDa. The enzyme presented higher activity at 50 °C and pH 5.0, using 100 mM citrate–phosphate buffer, was inhibited by Ag1+, Hg2+, Cu2+, Mg2+, and presented KM of 23.60 µM. Mature dextranase is composed of 585 amino acids residues, with a predicted molecular weight of 64.38 kDa and pI 5.96. This dextranase showed a strong phylogenetic similarity when compared to Trichoderma harzianum dextranase. Its structure consists of two domains: the first composed by 15 β strands, and the second composed by a right-handed parallel β-helix.
Palavras-chave: Enzyme
Verticillium chlamydosporium
Purification
3D structure
Editor: 3 Biotech
Tipo de Acesso: Springer-Verlag GmbH Germany, part of Springer Nature
URI: https://doi.org/10.1007/s13205-018-1192-4
http://www.locus.ufv.br/handle/123456789/24107
Data do documento: Mar-2018
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