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https://locus.ufv.br//handle/123456789/23770
Tipo: | Artigo |
Título: | Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.) |
Autor(es): | Rodrigues, Marcelo Costa, Thales H. F. Silva, Luzimar Campos Otoni, Wagner Campos Festucci-Buselli, Reginaldo Alves |
Abstract: | In vitro propagation of neem (Azadirachta indica A. Juss.) may offer an efficient alternative to seed propagation of this species. For optimization of in vitro propagation, different basal salt formulations, growth regulators, and culture container sealants (polytetrafluoroethylene hydrophobic membranes [PTFE]) were evaluated. Nodal segments cultured on Murashige and Skoog (MS) medium showed the highest shoot formation per explant (1.67). Explants cultured in flasks containing MS medium with 0.5 mg L−1 benzyladenine, 0.5 mg L−1 kinetin, and 0.05 mg L−1 naphthaleneacetic acid, and sealed with two PTFE membranes, produced the highest number of shoots (4.04). In contrast, explants cultured in flasks without membranes showed leaf chlorosis and senescence. For plant recovery, regenerants were acclimatized in a substrate of coconut fiber and eucalyptus bark (1:1) and showed 80% survival. Our results indicated that the use of flasks with vents was beneficial for in vitro propagation of this important plant. |
Palavras-chave: | Azadirachta indica Polytetrafluoroethylene membranes Plant growth regulators Micropropagation Gas exchange |
Editor: | In Vitro Cellular & Developmental Biology - Plant |
Tipo de Acesso: | The Society for In Vitro Biology |
URI: | https://doi.org/10.1007/s11627-011-9398-8 http://www.locus.ufv.br/handle/123456789/23770 |
Data do documento: | Fev-2012 |
Aparece nas coleções: | Artigos |
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artigo.pdf Until 2100-12-31 | Texto completo | 334,59 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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