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https://locus.ufv.br//handle/123456789/22692| Tipo: | Artigo |
| Título: | A practical and reliable procedure for in vitro induction of tetraploid tomato |
| Autor(es): | Praça, Milene Miranda Carvalho, Carlos Roberto de Clarindo, Wellington Ronildo |
| Abstract: | A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets. |
| Palavras-chave: | Colchicine Chromosome Flow cytometry Solanum lycopersicum |
| Editor: | Scientia Horticulturae |
| Tipo de Acesso: | 2009 Elsevier B.V. All rights reserved. |
| URI: | https://doi.org/10.1016/j.scienta.2009.05.032 http://www.locus.ufv.br/handle/123456789/22692 |
| Data do documento: | 1-Out-2009 |
| Aparece nas coleções: | Artigos |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| artigo.pdf Until 2100-12-31 | Texto completo | 175,83 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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