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https://locus.ufv.br//handle/123456789/21798
Tipo: | Artigo |
Título: | Diversity of soil-dwelling Trichoderma in Colombia and their potential as biocontrol agents against the phytopathogenic fungus Sclerotinia sclerotiorum (Lib.) de Bary |
Autor(es): | Smith, Alexander Beltrán, Camilo A. Kusunoki, Manabu Cotes, Alba M. Motohashi, Keiichi Kondo, Takumasa Deguchi, Michihito |
Abstract: | Twenty-one isolates of Trichoderma spp. were collected from eight states in Colombia and characterized based on the 5′ end of the translation elongation factor-1α (EF1-α1) gene and RNA polymerase II gene encoding the second largest protein subunit (RPB2) by using mixed primers. Seven species of soil-dwelling Trichoderma were found: T. atroviride, T. koningiopsis, T. asperellum, T. spirale, T. harzianum, T. brevicompactum and T. longibrachiatum. Species identifications based on the EF1-α1 gene were consistent with those obtained from the RPB2 gene. Phylogenetic analyses with high bootstrap values supported the validity of the identification of all isolates. These results suggest that using the combination of the genes EF1-α1 and RPB2 is highly reliable for molecular characterization of Trichoderma species. Trichoderma asperellum Th034, T. atroviride Th002 and T. harzianum Th203 prevented germination of more than 70 % of sclerotia of Sclerotinia sclerotiorum in bioassay tests and are promising biological control agents. No relationship between mycelium growth rate and parasitism level was found. |
Palavras-chave: | Trichoderma spp. EF1-α1 gene RPB2 gene Molecular phylogeny Biological control Sclerotinia sclerotiorum |
Editor: | Journal of General Plant Pathology |
Tipo de Acesso: | The Phytopathological Society of Japan and Springer Japan |
URI: | https://doi.org/10.1007/s10327-012-0419-1 http://www.locus.ufv.br/handle/123456789/21798 |
Data do documento: | 24-Nov-2012 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 611,57 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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