Use este identificador para citar ou linkar para este item:
https://locus.ufv.br//handle/123456789/19892
Tipo: | Artigo |
Título: | DNA interaction with hoechst 33258: stretching experiments decouple the different binding modes |
Autor(es): | Silva, E. F. Ramos, E. B. Rocha, M. S. |
Abstract: | By performing single molecule stretching experiments with optical tweezers, we have studied the DNA interaction with the ligand Hoechst 33258. The mechanical properties of the complexes formed as a function of ligand concentration were directly determined from these measurements by fitting the force × extension curve to the WormLike Chain model of semiflexible polymers. In addition, the physicochemical parameters of the interaction were extracted from the persistence length data by using a previously developed two-sites quenched disorder statistical model, allowing the determination of the binding isotherm. Such approach has allowed us to decouple the two different binding modes present in this system. In particular, it was found that the binding isotherm consists of two Hill-type processes, one noncooperative and the other strongly cooperative. Finally, DNA condensation due to the interaction with the ligand was also verified and characterized here by analyzing the apparent contour length of the complexes. |
Palavras-chave: | Hoechst 33258 Different Binding Modes |
Editor: | The Journal of Physical Chemistry B |
Tipo de Acesso: | American Chemical Society |
URI: | https://doi.org/10.1021/jp403945e http://www.locus.ufv.br/handle/123456789/19892 |
Data do documento: | 28-Mai-2013 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo.pdf Until 2100-12-31 | Texto completo | 935,71 kB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.