Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19650
Tipo: Artigo
Título: A central composite rotatable design (CCRD) approach to study the combined effect of antimicrobial agents against bacterial pathogens
Autor(es): Santos, Fernanda Godoy
Mendonça, Layanne Andrade
Mantovani, Hilário Cuquetto
Abstract: The combination of antimicrobial agents has been proposed as a therapeutic strategy to control bacterial diseases and to reduce the emergence of antibiotic-resistant strains in clinical environments. In this study, the interaction between the lantibiotic bovicin HC5 with chloramphenicol, gentamicin, nisin, lysostaphin and hydrogen peroxide against Staphylococcus aureus O46 was evaluated by MIC assays. The central composite rotatable design (CCRD), a robust and economic statistical design, was used to combine concentration levels of different antimicrobials agents with distinct mechanisms of action and the presence of significant interactions among the antimicrobials was determined by regression analysis. According to the adjusted model, there were no significant interactions between bovicin HC5 and gentamicin, lysostaphin, nisin or hydrogen peroxide. However, bovicin HC5 showed a significant interaction (P < 0.02) with chloramphenicol. This is the first study applying the CCRD approach to evaluate the combined effect of antimicrobials against S. aureus. Based on our results, this approach is an effective strategy to determine synergistic interactions between antimicrobial agents applied in human and veterinary medicine against bacterial pathogens.
Palavras-chave: Bacteriocin
Bovicin HC5
Chloramphenicol
Synergism
Factorial design
Editor: World Journal of Microbiology and Biotechnology
Tipo de Acesso: Springer Science+Business Media Dordrecht
URI: http://dx.doi.org/10.1007/s11274-015-1884-4
http://www.locus.ufv.br/handle/123456789/19650
Data do documento: 17-Jun-2015
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdf
  Until 2100-12-31
texto completo426,73 kBAdobe PDFVisualizar/Abrir ACESSO RESTRITO


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.