Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19634
Tipo: Artigo
Título: The Linum usitatissimum L. plastome reveals atypical structural evolution, new editing sites, and the phylogenetic position of Linaceae within Malpighiales
Autor(es): Santana Lopes, Amanda de
Pacheco, Túlio Gomes
Santos, Karla Gasparini dos
Vieira, Leila do Nascimento
Guerra, Miguel Pedro
Nodari, Rubens Onofre
Souza, Emanuel Maltempi de
Pedrosa, Fábio de Oliveira
Rogalski, Marcelo
Abstract: Flax (Linum usitatissimum L.) is an economically important crop used as food, feed, and industrial feedstock. It belongs to the Linaceae family, which is noted by high morphological and ecological diversity. Here, we reported the complete sequence of flax plastome, the first species within Linaceae family to have the plastome sequenced, assembled and characterized in detail. The plastome of flax is a circular DNA molecule of 156,721 bp with a typical quadripartite structure including two IRs of 31,990 bp separating the LSC of 81,767 bp and the SSC of 10,974 bp. It shows two expansion events from IRB to LSC and from IRB to SSC, and a contraction event in the IRA-LSC junction, which changed significantly the size and the gene content of LSC, SSC and IRs. We identified 109 unique genes and 2 pseudogenes (rpl23 and ndhF). The plastome lost the conserved introns of clpP gene and the complete sequence of rps16 gene. The clpP, ycf1, and ycf2 genes show high nucleotide and aminoacid divergence, but they still possibly retain the functionality. Moreover, we also identified 176 SSRs, 20 tandem repeats, and 39 dispersed repeats. We predicted in 18 genes a total of 53 RNA editing sites of which 32 were not found before in other species. The phylogenetic inference based on 63 plastid protein-coding genes of 38 taxa supports three major clades within Malpighiales order. One of these clades has flax (Linaceae) sister to Chrysobalanaceae family, differing from earlier studies that included Linaceae into the euphorbioid clade.
Palavras-chave: Extranuclear inheritance
Rearrangements
Gene duplication
Plastid microsatellites
Plastid evolution
Editor: Plant Cell Reports
Tipo de Acesso: Springer-Verlag GmbH Germany
URI: http://dx.doi.org/10.1007/s00299-017-2231-z
http://www.locus.ufv.br/handle/123456789/19634
Data do documento: 30-Out-2017
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