Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19500
Tipo: Artigo
Título: Diversity and distribution of the endophytic bacterial community at different stages of Eucalyptus growth
Autor(es): Miguel, Paulo Sérgio Balbino
Neves, Júlio César Lima
Borges, Arnaldo Chaer
Tótola, Marcos Rogério
Costa, Maurício Dutra
Oliveira, Marcelo Nagem Valério de
Delvaux, Júlio César
Jesus, Guilherme Luiz de
Abstract: The relationships between plants and endophytic bacteria significantly contribute to plant health and yield. However, the microbial diversity in leaves of Eucalyptus spp. is still poorly characterized. Here, we investigated the endophytic diversity in leaves of hybrid Eucalyptus grandis x E. urophylla (Eucalyptus “urograndis”) by using culture-independent and culture-dependent approaches, to better understand their ecology in leaves at different stages of Eucalyptus development, including bacteria with N2 fixation potential. Firmicutes, Proteobacteria (classes alpha-, beta- and gamma-) and Actinobacteria were identified in the Eucalyptus “urograndis” endophytic bacterial community. Within this community, the species Novosphingobium barchaimii, Rhizobium grahamii, Stenotrophomonas panacihumi, Paenibacillus terrigena, P. darwinianus and Terrabacter lapilli represent the first report these bacteria as endophytes. The diversity of the total endophytic bacteria was higher in the leaves from the ‘field’ (the Shannon–Wiener index, 2.99), followed by the indices obtained in the ‘clonal garden’ (2.78), the ‘recently out from under shade (2.68), ‘under shade’ (2.63) and ‘plants for dispatch’ (2.51). In contrast, for diazotrophic bacteria, the highest means of these indices were obtained from the leaves of plants in the ‘under shade’ (2.56), ‘recently out from under shade (2.52)’ and ‘field’ stages (2.54). The distribution of the endophytic bacterial species in Eucalyptus was distinct and specific to the development stages under study, and many of the species had the potential for nitrogen fixation, raising the question of whether these bacteria could contribute to overall nitrogen metabolism of Eucalyptus.
Palavras-chave: Diversity
Endophytic bacteria
DGGE
Sequencing
Eucalyptus development stages
Editor: Antonie van Leeuwenhoek
Tipo de Acesso: Springer International Publishing
URI: http://dx.doi.org/10.1007/s10482-016-0676-7
http://www.locus.ufv.br/handle/123456789/19500
Data do documento: 24-Mar-2016
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdf
  Until 2100-12-31
Texto completo1,37 MBAdobe PDFVisualizar/Abrir ACESSO RESTRITO


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.