Use este identificador para citar ou linkar para este item: https://locus.ufv.br//handle/123456789/19031
Tipo: Artigo
Título: Ploidy variation in kluyveromyces marxianus separates dairy and non-dairy isolates
Autor(es): Merino, Raúl A. Ortiz
Varela, Javier A.
Coughlan, Aisling Y.
Hoshida, Hisashi
Silveira, Wendel B. da
Wilde, Caroline
Kuijpers, Niels G. A.
Geertman, Jan Maarten
Wolfe, Kenneth H.
Morrissey, John P.
Abstract: Kluyveromyces marxianus is traditionally associated with fermented dairy products, but can also be isolated from diverse non-dairy environments. Because of thermotolerance, rapid growth and other traits, many different strains are being developed for food and industrial applications but there is, as yet, little understanding of the genetic diversity or population genetics of this species. K. marxianus shows a high level of phenotypic variation but the only phenotype that has been clearly linked to a genetic polymorphism is lactose utilisation, which is controlled by variation in the LAC12 gene. The genomes of several strains have been sequenced in recent years and, in this study, we sequenced a further nine strains from different origins. Analysis of the Single Nucleotide Polymorphisms (SNPs) in 14 strains was carried out to examine genome structure and genetic diversity. SNP diversity in K. marxianus is relatively high, with up to 3% DNA sequence divergence between alleles. It was found that the isolates include haploid, diploid, and triploid strains, as shown by both SNP analysis and flow cytometry. Diploids and triploids contain long genomic tracts showing loss of heterozygosity (LOH). All six isolates from dairy environments were diploid or triploid, whereas 6 out 7 isolates from non-dairy environment were haploid. This also correlated with the presence of functional LAC12 alleles only in dairy haplotypes. The diploids were hybrids between a non-dairy and a dairy haplotype, whereas triploids included three copies of a dairy haplotype.
Palavras-chave: lactose transport
Non-conventional yeast
Yeast evolution
Industrial yeast
Dairy
Kluyveromyces
LAC12
Editor: Frontiers in Genetics
Tipo de Acesso: Open Access
URI: https://doi.org/10.3389/fgene.2018.00094
http://www.locus.ufv.br/handle/123456789/19031
Data do documento: 5-Mar-2018
Aparece nas coleções:Artigos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
artigo.pdfartigo5,78 MBAdobe PDFThumbnail
Visualizar/Abrir


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.