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https://locus.ufv.br//handle/123456789/18792
Tipo: | Artigo |
Título: | Termiticidal lectins from Myracrodruon urundeuva (Anacardiaceae) cause midgut damage when ingested by Nasutitermes corniger (Isoptera: Termitidae) workers |
Autor(es): | Lima, Thâmarah A. Fernandes, Kenner M. Oliveira, Ana Patrícia S. Dornelles, Leonardo P. Martins, Gustavo F. Napoleão, Thiago H. Paiva, Patrícia M.G. |
Abstract: | Myracrodruon urundeuva is a hardwood tree, and its bark, heartwood and leaf contain lectins (MuBL, MuHL and MuLL respectively) with termiticidal activity against Nasutitermes corniger. In this work, the effects of these lectins on the midgut of N. corniger workers were evaluated. The insects were supplied with an artificial diet containing the lectins at their respective LC 50 (previously determined). At 48 h after treatment, the midguts were dissected and fixed for histopathology analyses. Toluidine-blue-stained midguts from lectin-treated workers showed disorganisation, with the presence of debris in the lumen and the absence of brush border. Fluorescence microscopy revealed that the numbers of digestive and proliferating cells were lower in lectin-treated individuals than in the control, and caspase-3 staining confirmed the occurrence of cell apoptosis. Enteroendocrine cells were not seen in the treated individuals. The midguts from treated insects showed greater staining for peroxidase than the control, suggesting that the lectins caused oxidative stress. Staining with wheat germ agglutinin conjugated to FITC revealed that the lectins interfered with the integrity of the peritrophic matrix. This study showed that termiticidal lectins from M. urundeuva cause severe injuries, oxidative stress and cell death in the midgut of N. corniger workers. |
Palavras-chave: | Insecticidal activity Midgut epithelium Peritrophic matrix Apoptosis Hardwood tree |
Editor: | Pest Management Science |
Tipo de Acesso: | Society of Chemical Industry |
URI: | https://doi.org/10.1002/ps.4415 http://www.locus.ufv.br/handle/123456789/18792 |
Data do documento: | 16-Ago-2016 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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artigo.pdf Until 2100-12-31 | Texto completo | 1,08 MB | Adobe PDF | Visualizar/Abrir ACESSO RESTRITO |
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